Melanoma cells were prepared from the biopsy of a lung metastasis of minipiglet, 2 and a half months old, with spontaneous cutaneous melanoma and metastasis in lymph nodes, lung, spleen, and liver. 48hs culture of tumoral cells resulted in melanocytic bipolar and pigmented cells.
Treatment protocol
Gentamicine, penicilline, steptomycin and fungizone treatment of the biopsies before and during the primary culture.
Growth protocol
Conditions for primary culture of pig melanocytic cells were described in Julé et al, Pigment Cell Research 16:407-410, 2003.
Extracted molecule
polyA RNA
Extraction protocol
Dynabead mRNA direct kit
Description
Library was generated using the SAGE adaptation for downsized extracts (SADE) method (Virlon et al, P.N.A.S. 96:15286-15291, 1999). Cell lysates were centrifugated to discard melanin.
