|
| Status |
Public on Jun 03, 2014 |
| Title |
Acrolein-2 |
| Sample type |
RNA |
| |
|
| Source name |
HUVEC
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: umbilical vein endothelial cells
|
| Treatment protocol |
Cells were grown in media containing 0.1% serum overnight, incubated with 10uM acrolein or vehicle in HBSS for 2 hr, and allowed to recover in normal media for 4hr.
|
| Growth protocol |
HUVEC in log phase growth (passage 3-5) were maintained in EBM media (Lonza).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Qiagen kit
|
| Label |
Cy3
|
| Label protocol |
100ng total RNA treated with CIP, denatured, and ligated to pCP-Cy3 with T4 RNA ligase, then purified on spin column, dried with Speed-vac
|
| |
|
| Hybridization protocol |
resuspended RNA hybirdized to array in hybridization buffer at 550C for 20hr. Then slides washed in Agilent wash buffers 1 and 2.
|
| Scan protocol |
scanned with Agilent microarray scanner (G2505B); one color images extracted with Feature Extraction Software (v9.5.1 Agilent)
|
| Description |
miRNA expression at 4hours after treatement
|
| Data processing |
raw data files extracted from Agilent Feature extraction software were processed and analyzed with GeneSpring GX9.0 software
|
| |
|
| Submission date |
Apr 14, 2014 |
| Last update date |
Jun 03, 2014 |
| Contact name |
Timothy O'Toole |
| Organization name |
University of Louisville
|
| Street address |
302E. Muhammad Ali Blvd
|
| City |
Louisville |
| State/province |
KY |
| ZIP/Postal code |
40202 |
| Country |
USA |
| |
|
| Platform ID |
GPL8617 |
| Series (1) |
| GSE56782 |
Acrolein-mediated changes in HUVEC miRNA |
|
