|
Status |
Public on May 30, 2014 |
Title |
JAG1-treatment |
Sample type |
RNA |
|
|
Source name |
HMEC cells
|
Organism |
Homo sapiens |
Characteristics |
treatment: JAG1 cell type: human mammary epithelial cells
|
Treatment protocol |
HMEC cells were treated with 40mM of Jagged1 active peptide or control peptide (Anaspec) for 24hrs.
|
Growth protocol |
Primary human mammary epithelial cells (HMECs), purchased from Lonza Inc. were cultured in MEGM Mammary Epithelial Cell Growth Medium.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted by Rneasy mini plus kit followed by Dnase I treatment.
|
Label |
SYBR Green
|
Label protocol |
SYBR-ROX PCR assays were performed using specific primers for mature miRNAs (SA Biosciences) following the manufacturer’s instructions.
|
|
|
Hybridization protocol |
n/a
|
Scan protocol |
n/a
|
Description |
Test
|
Data processing |
Normalization and data analysis were performed according to the manufacturer’using their web-based software package: http://sabiosciences.com/pcrarraydataanalysis.php
|
|
|
Submission date |
Apr 15, 2014 |
Last update date |
May 30, 2014 |
Contact name |
AC CHANG |
Organization name |
RPCI
|
Street address |
Elm and Carlton
|
City |
Buffalo |
ZIP/Postal code |
14209 |
Country |
USA |
|
|
Platform ID |
GPL18572 |
Series (1) |
GSE56801 |
Jagged1 regulates miRNA expression profile |
|