|
| Status |
Public on May 30, 2014 |
| Title |
JAG1-treatment |
| Sample type |
RNA |
| |
|
| Source name |
HMEC cells
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: JAG1
cell type: human mammary epithelial cells
|
| Treatment protocol |
HMEC cells were treated with 40mM of Jagged1 active peptide or control peptide (Anaspec) for 24hrs.
|
| Growth protocol |
Primary human mammary epithelial cells (HMECs), purchased from Lonza Inc. were cultured in MEGM Mammary Epithelial Cell Growth Medium.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by Rneasy mini plus kit followed by Dnase I treatment.
|
| Label |
SYBR Green
|
| Label protocol |
SYBR-ROX PCR assays were performed using specific primers for mature miRNAs (SA Biosciences) following the manufacturer’s instructions.
|
| |
|
| Hybridization protocol |
n/a
|
| Scan protocol |
n/a
|
| Description |
Test
|
| Data processing |
Normalization and data analysis were performed according to the manufacturer’using their web-based software package: http://sabiosciences.com/pcrarraydataanalysis.php
|
| |
|
| Submission date |
Apr 15, 2014 |
| Last update date |
May 30, 2014 |
| Contact name |
AC CHANG |
| Organization name |
RPCI
|
| Street address |
Elm and Carlton
|
| City |
Buffalo |
| ZIP/Postal code |
14209 |
| Country |
USA |
| |
|
| Platform ID |
GPL18572 |
| Series (1) |
| GSE56801 |
Jagged1 regulates miRNA expression profile |
|
