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Status
Public on Mar 31, 2007
Title
HSC ltbp1-/- vs wt 85
Sample type
RNA
Channel 1 Source name
Hepatic stellate cells (HSC)_wt_activated_1
Organism
Mus musculus Characteristics
HSC, wt, activated (by in vitro culture on plastic for 6 days), pooled cells of 5 animals
Extracted molecule
total RNA
Label
Cy3
Channel 2 Source name
Hepatic stellate cells (HSC)_ltbp1-/-_activated_1
Organism
Mus musculus Characteristics
HSC, ltbp1-/-, activated (by in vitro culture on plastic for 6 days), pooled cells of 5 animals
Extracted molecule
total RNA
Label
Cy5
Scan protocol
Image capture and signal quantification were done with the Microarray Scanner G2505B (Agilent Technologies) and ImaGene software version 5.0 (BioDiscovery, Los Angeles, MA)
Description
1 µg of linear amplified RNA from wt and ltbp1-/- in vitro activated HSC (6d culture on plastic) were labelled by reverse transcription with Cy3 and Cy5 fluorescence, respectively. Cy3 and Cy5 labeled cDNA were combined and subdivided into two parts to perform co-hybridizations with the PIQOR Immunology Microarray, mouse
Data processing
PubmedID
Submission date
Sep 21, 2006
Last update date
Dec 12, 2006
Contact name
Sebastian Knoebel
E-mail(s)
sebastian@miltenyibiotec.de Phone
0221-95048220
Fax
0221-9504848
URL
http://www.miltenyibiotec.com Organization name
MiltenyiBiotec GmbH
Street address
Nattermannallee 1
City
Cologne
ZIP/Postal code
50829
Country
Germany
Platform ID
GPL4363 Series (1)
GSE5670 Hepatic stellate cells (HSC), quiescent and activated - influence of ltbp1-k.o. on activation in vitro
Data table header descriptions ID_REF CH1_int_mean Channel 1 mean spot intensity
CH1_int_bkg Channel 1 mean background intensity
CH2_int_mean Channel 2 mean spot intensity
CH2_int_bkg Channel 2 mean background intensity
Ch1_SIG_MBKG Channel 1 background corrected and normalized spot intensity
Ch2_SIG_MBKG Channel 2 background corrected and normalized spot intensity
VALUE normalized log2 ratio defined as Ch2/Ch1
Flag 0 denotes satisfactory feature while >0 denotes features that did not meet
Data table ID_REF CH1_int_mean CH1_int_bkg CH2_int_mean CH2_int_bkg Ch1_SIG_MBKG Ch2_SIG_MBKG VALUE Flag 1
13235
94
26778
81
3
2
433
80
677
74
450
473
0.071
0
3
279
81
340
74
250
210
-0.25
0
4
565
79
1317
72
625
968
0.631
0
5
591
75
1038
69
662
755
0.188
0
6
139
74
164
68
81
77
-0.066
0
7
884
73
1124
66
1047
820
-0.353
0
8
111
75
110
68
0
9
333
76
477
69
326
322
-0.019
0
10
300
73
383
67
3
11
171
72
308
67
125
191
0.61
0
12
919
88
1764
76
1078
1302
0.272
0
13
289
80
454
73
265
301
0.187
0
14
18041
82
29190
76
24874
21020
-0.243
0
15
308
77
433
70
292
287
-0.029
0
16
163
76
215
68
109
117
0.096
0
17
192
74
238
67
149
135
-0.136
0
18
314
72
386
66
306
253
-0.276
0
19
110
72
109
66
0
20
90
71
87
67
0
4304 143 Kbytes .Supplementary data files not provided
