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Status
Public on Mar 31, 2007
Title
HSC ltbp1-/- vs wt 90
Sample type
RNA
Channel 1 Source name
Hepatic stellate cells (HSC)_wt_activated_2
Organism
Mus musculus Characteristics
HSC, wt, activated (by in vitro culture on plastic for 6 days), pooled cells of 5 animals
Extracted molecule
total RNA
Label
Cy3
Channel 2 Source name
Hepatic stellate cells (HSC)_ltbp1-/-_activated_2
Organism
Mus musculus Characteristics
HSC, ltbp1-/-, activated (by in vitro culture on plastic for 6 days), pooled cells of 5 animals
Extracted molecule
total RNA
Label
Cy5
Scan protocol
Image capture and signal quantification were done with the Microarray Scanner G2505B (Agilent Technologies) and ImaGene software version 5.0 (BioDiscovery, Los Angeles, MA)
Description
1 µg of linear amplified RNA from wt and ltbp1-/- in vitro activated HSC (6d culture on plastic) were labelled by reverse transcription with Cy3 and Cy5 fluorescence, respectively. Cy3 and Cy5 labeled cDNA were combined and subdivided into two parts to perform co-hybridizations with the PIQOR Immunology Microarray, mouse
Data processing
PubmedID
Submission date
Sep 21, 2006
Last update date
Dec 12, 2006
Contact name
Sebastian Knoebel
E-mail(s)
sebastian@miltenyibiotec.de Phone
0221-95048220
Fax
0221-9504848
URL
http://www.miltenyibiotec.com Organization name
MiltenyiBiotec GmbH
Street address
Nattermannallee 1
City
Cologne
ZIP/Postal code
50829
Country
Germany
Platform ID
GPL4363 Series (1)
GSE5670 Hepatic stellate cells (HSC), quiescent and activated - influence of ltbp1-k.o. on activation in vitro
Data table header descriptions ID_REF CH1_int_mean Channel 1 mean spot intensity
CH1_int_bkg Channel 1 mean background intensity
CH2_int_mean Channel 2 mean spot intensity
CH2_int_bkg Channel 2 mean background intensity
Ch1_SIG_MBKG Channel 1 background corrected and normalized spot intensity
Ch2_SIG_MBKG Channel 2 background corrected and normalized spot intensity
VALUE normalized log2 ratio defined as Ch2/Ch1
Flag 0 denotes satisfactory feature while >0 denotes features that did not meet
Data table ID_REF CH1_int_mean CH1_int_bkg CH2_int_mean CH2_int_bkg Ch1_SIG_MBKG Ch2_SIG_MBKG VALUE Flag 1
21699
93
42502
96
0
2
1062
84
2233
88
1517
1383
-0.134
0
3
510
84
986
91
665
573
-0.215
0
4
1471
79
6146
90
2158
3907
0.856
0
5
1617
84
3743
100
2374
2353
-0.013
0
6
257
84
439
101
274
213
-0.363
0
7
1913
81
4052
100
2838
2551
-0.154
0
8
128
79
229
98
79
81
0.037
0
9
642
78
1225
96
879
724
-0.279
0
10
769
78
1608
96
1074
973
-0.144
0
11
330
79
945
94
392
545
0.474
0
12
2230
92
5148
91
3317
3259
-0.025
0
13
544
85
1138
89
716
672
-0.092
0
14
27400
83
49217
92
42608
31495
-0.436
0
15
588
78
1166
91
796
689
-0.207
0
16
328
82
711
99
385
391
0.023
0
17
253
84
475
102
268
236
-0.184
0
18
635
81
1075
100
864
625
-0.467
0
19
167
81
301
99
138
126
-0.14
0
20
106
80
169
97
0
4304 151 Kbytes .Supplementary data files not provided
