|
| Status |
Public on Mar 31, 2007 |
| Title |
HSC activated vs quiescent 40 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Hepatic stellate cells (HSC)_wt_quiescent_1
|
| Organism |
Mus musculus |
| Characteristics |
HSC, wt, quiescent (cultured in vitro on plastic for 1 day), pooled cells of 5 animals
|
| Extracted molecule |
total RNA |
| Label |
Cy3
|
| |
|
| Channel 2 |
| Source name |
Hepatic stellate cells (HSC)_wt_quiescent_3
|
| Organism |
Mus musculus |
| Characteristics |
HSC, wt, activated (by in vitro culture on plastic for 6 days), pooled cells of 5 animals
|
| Extracted molecule |
total RNA |
| Label |
Cy5
|
| |
|
| |
| Scan protocol |
Image capture and signal quantification were done with the Microarray Scanner G2505B (Agilent Technologies) and ImaGene software version 5.0 (BioDiscovery, Los Angeles, MA)
|
| Description |
1 µg of linear amplified RNA from quiescent and in vitro activated wt HSC (6d culture on plastic) were labelled by reverse transcription with Cy3 and Cy5 fluorescence, respectively. Cy3 and Cy5 labeled cDNA were combined and subdivided into two parts to perform co-hybridizations with the PIQOR Immunology Microarray, mouse
|
| Data processing |
PubmedID
|
| |
|
| Submission date |
Sep 21, 2006 |
| Last update date |
Dec 12, 2006 |
| Contact name |
Sebastian Knoebel |
| E-mail(s) |
sebastian@miltenyibiotec.de
|
| Phone |
0221-95048220
|
| Fax |
0221-9504848
|
| URL |
http://www.miltenyibiotec.com
|
| Organization name |
MiltenyiBiotec GmbH
|
| Street address |
Nattermannallee 1
|
| City |
Cologne |
| ZIP/Postal code |
50829 |
| Country |
Germany |
| |
|
| Platform ID |
GPL4363 |
| Series (1) |
| GSE5670 |
Hepatic stellate cells (HSC), quiescent and activated - influence of ltbp1-k.o. on activation in vitro |
|
| Data table header descriptions |
| ID_REF |
|
| CH1_int_mean |
Channel 1 mean spot intensity |
| CH1_int_bkg |
Channel 1 mean background intensity |
| CH2_int_mean |
Channel 2 mean spot intensity |
| CH2_int_bkg |
Channel 2 mean background intensity |
| Ch1_SIG_MBKG |
Channel 1 background corrected and normalized spot intensity |
| Ch2_SIG_MBKG |
Channel 2 background corrected and normalized spot intensity |
| VALUE |
normalized log2 ratio defined as Ch2/Ch1 |
| Flag |
0 denotes satisfactory feature while >0 denotes features that did not meet |
