|
| Status |
Public on Sep 30, 2016 |
| Title |
FOM217-1 iMsx1 -Dox |
| Sample type |
RNA |
| |
|
| Source name |
melanocytes
|
| Organism |
Homo sapiens |
| Characteristics |
individual: donor 1 (FOM217-1)
cell type: foreskin-derived melanocytes
infection: inducible MSX1 lentiviral vector (iMsx1)
treatment: no doxycycline
time: 0hr
|
| Treatment protocol |
Human melanocytes were treated with 500 ng/ml doxycycline (+Dox) or control PBS (-Dox) for 6h.
|
| Growth protocol |
Human melanocytes were grown in 254CF medium before they reach the 70% confluency.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were washed again with HBSS and lysed directly in TRI Reagent® RNA Isolation Reagent (Sigma) and stored at -20°C.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
iMsx1 vector expression and no doxycycline
|
| Data processing |
The data were normalized using quantile normalization with lumi in R
|
| |
|
| Submission date |
Apr 22, 2014 |
| Last update date |
Sep 30, 2016 |
| Contact name |
Mizuho Fukunaga-Kalabis |
| E-mail(s) |
mkalabis@wistar.org
|
| Organization name |
The Wistar Institute
|
| Street address |
3601 Spruce Street
|
| City |
Philadelphia |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE56957 |
MSX1-induced neural crest-like phenotype in human melanocytes |
|
