|
Status |
Public on Sep 30, 2016 |
Title |
FOM100811-1 iMsx1 -Dox |
Sample type |
RNA |
|
|
Source name |
melanocytes
|
Organism |
Homo sapiens |
Characteristics |
individual: donor 2 (FOM100811-1) cell type: foreskin-derived melanocytes infection: inducible MSX1 lentiviral vector (iMsx1) treatment: no doxycycline time: 0hr
|
Treatment protocol |
Human melanocytes were treated with 500 ng/ml doxycycline (+Dox) or control PBS (-Dox) for 6h.
|
Growth protocol |
Human melanocytes were grown in 254CF medium before they reach the 70% confluency.
|
Extracted molecule |
total RNA |
Extraction protocol |
Cells were washed again with HBSS and lysed directly in TRI Reagent® RNA Isolation Reagent (Sigma) and stored at -20°C.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
iMsx1 vector expression and no doxycycline
|
Data processing |
The data were normalized using quantile normalization with lumi in R
|
|
|
Submission date |
Apr 22, 2014 |
Last update date |
Sep 30, 2016 |
Contact name |
Mizuho Fukunaga-Kalabis |
E-mail(s) |
mkalabis@wistar.org
|
Organization name |
The Wistar Institute
|
Street address |
3601 Spruce Street
|
City |
Philadelphia |
ZIP/Postal code |
19104 |
Country |
USA |
|
|
Platform ID |
GPL10558 |
Series (1) |
GSE56957 |
MSX1-induced neural crest-like phenotype in human melanocytes |
|