|
| Status |
Public on Jan 01, 2016 |
| Title |
IREB2 knock-out Male 2 |
| Sample type |
RNA |
| |
|
| Source name |
IREB2 knock-out_male_lung
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57/BL6
genotype/variation: IREB2 Knockout (Irp2-/-)
gender: male
age: ~28-32 weeks
tissue: lung
|
| Treatment protocol |
none
|
| Growth protocol |
Mice were housed in the same animal facility with access to same food and water.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated according to Qiagen RNAeasy Kit per manufacturers protocol
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 3000 7G
|
| Description |
KM2
|
| Data processing |
The data were analyzed using the Bioconductor package in R to perform RMA normalization.
|
| |
|
| Submission date |
Apr 24, 2014 |
| Last update date |
Jan 01, 2016 |
| Contact name |
Augustine MK Choi |
| Organization name |
Weill Cornell Medical College
|
| Department |
Department of Medicine
|
| Street address |
525 East 68th Street, Room M-522, Box 130
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL17777 |
| Series (1) |
| GSE57048 |
Irp2 mediates cigarette smoke-induced bronchitis and emphysema via regulation of cytochrome c oxidase and mitochondrial iron loading. |
|
