|
Status |
Public on Jan 01, 2016 |
Title |
IREB2 knock-out Female 3 |
Sample type |
RNA |
|
|
Source name |
IREB2 knock-out_female_lung
|
Organism |
Mus musculus |
Characteristics |
strain background: C57/BL6 genotype/variation: IREB2 Knockout (Irp2-/-) gender: female age: ~28-32 weeks tissue: lung
|
Treatment protocol |
none
|
Growth protocol |
Mice were housed in the same animal facility with access to same food and water.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was isolated according to Qiagen RNAeasy Kit per manufacturers protocol
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
|
|
Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 3000 7G
|
Description |
KF3
|
Data processing |
The data were analyzed using the Bioconductor package in R to perform RMA normalization.
|
|
|
Submission date |
Apr 24, 2014 |
Last update date |
Jan 01, 2016 |
Contact name |
Augustine MK Choi |
Organization name |
Weill Cornell Medical College
|
Department |
Department of Medicine
|
Street address |
525 East 68th Street, Room M-522, Box 130
|
City |
New York |
State/province |
NY |
ZIP/Postal code |
10065 |
Country |
USA |
|
|
Platform ID |
GPL17777 |
Series (1) |
GSE57048 |
Irp2 mediates cigarette smoke-induced bronchitis and emphysema via regulation of cytochrome c oxidase and mitochondrial iron loading. |
|