|
| Status |
Public on Sep 18, 2014 |
| Title |
Liver_HighGlucoseHS46_genotype |
| Sample type |
genomic |
| |
|
| Source name |
heterogeneous stock rat tail
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: heterogeneous stock
phenotype: High Glucose
tissue: tail
gender: male
|
| Treatment protocol |
Rats were fasted overnight prior to being euthanized.
|
| Growth protocol |
Male rats were maintained two per cage and fed ad libitum. Rats were euthanized at 17 weeks of age after an overnight fast.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted using the Qiagen DNeasy kit
|
| Label |
biotin
|
| Label protocol |
Targeted genotyping technology was used. Two PCR reactions occur: the first is to amplify the targeted region and the second is to label the region for subsequent detection.
|
| |
|
| Hybridization protocol |
The Affymetrix 10K SNP array was selected for these studies for its overall comprehensive coverage. Samples are hybridized overnight.
|
| Scan protocol |
After hybridization, arrays were washed and sarrays are washed and loaded onto the Affymetrix GeneChip Scanner 3000
|
| Description |
Genome-wide 10K SNP array in heterogeneous stock rats
|
| Data processing |
All SNPs were given a call rate. Those that did not reach a sufficient call rate were removed.
|
| |
|
| Submission date |
Apr 26, 2014 |
| Last update date |
Sep 18, 2014 |
| Contact name |
Leah C Solberg Woods |
| E-mail(s) |
lsolberg@mcw.edu
|
| Phone |
414-955-2354
|
| Organization name |
Medical College of Wisconsin
|
| Department |
Pediatrics
|
| Street address |
8701 Watertown Plank Road
|
| City |
Milwaukee |
| State/province |
WI |
| ZIP/Postal code |
53226 |
| Country |
USA |
| |
|
| Platform ID |
GPL18612 |
| Series (2) |
| GSE57117 |
Genome-wide 10K SNP profiles in heterogeneous stock rats that differ in glucose tolerance |
| GSE57118 |
Heterogeneous stock rats that differ in glucose tolerance |
|
