|
Status |
Public on Sep 18, 2014 |
Title |
M520/N_Genotype |
Sample type |
genomic |
|
|
Source name |
heterogeneous stock founder rat tail
|
Organism |
Rattus norvegicus |
Characteristics |
strain: M520/N tissue: tail gender: male
|
Treatment protocol |
Rats were fasted overnight prior to being euthanized.
|
Growth protocol |
Male rats were maintained two per cage and fed ad libitum. Rats were euthanized at 17 weeks of age after an overnight fast.
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA was extracted using the Qiagen DNeasy kit
|
Label |
biotin
|
Label protocol |
Targeted genotyping technology was used. Two PCR reactions occur: the first is to amplify the targeted region and the second is to label the region for subsequent detection.
|
|
|
Hybridization protocol |
The Affymetrix 10K SNP array was selected for these studies for its overall comprehensive coverage. Samples are hybridized overnight.
|
Scan protocol |
After hybridization, arrays were washed and sarrays are washed and loaded onto the Affymetrix GeneChip Scanner 3000
|
Description |
Genome-wide 10K SNP array in heterogeneous stock rats
|
Data processing |
All SNPs were given a call rate. Those that did not reach a sufficient call rate were removed.
|
|
|
Submission date |
Apr 26, 2014 |
Last update date |
Sep 18, 2014 |
Contact name |
Leah C Solberg Woods |
E-mail(s) |
lsolberg@mcw.edu
|
Phone |
414-955-2354
|
Organization name |
Medical College of Wisconsin
|
Department |
Pediatrics
|
Street address |
8701 Watertown Plank Road
|
City |
Milwaukee |
State/province |
WI |
ZIP/Postal code |
53226 |
Country |
USA |
|
|
Platform ID |
GPL18612 |
Series (2) |
GSE57117 |
Genome-wide 10K SNP profiles in heterogeneous stock rats that differ in glucose tolerance |
GSE57118 |
Heterogeneous stock rats that differ in glucose tolerance |
|