|
| Status |
Public on May 06, 2014 |
| Title |
Human_BTH_147-A51-25739T |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
gastric cancer frozen tumor tissues
|
| Organism |
Homo sapiens |
| Characteristics |
gender: M
tissue: gastric
phenotype: gastric cancer
|
| Growth protocol |
Tumor tissues were collected after surgical resection and frozed for DNA extraction
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted using Gentra Puregene Cell Kit (Qiagen) following manufacturer’s instructions. QC process was performed using 1% agarose gel at 120V for 45min and single clear band with its size ~4000bp was defined as QC pass.
|
| Label |
Cy5
|
| Label protocol |
2 µg of genomic DNA were primed with 5µl random DNA primer at 96°C for 5 min
|
| |
|
| Channel 2 |
| Source name |
standard reference genomic DNA
|
| Organism |
Homo sapiens |
| Characteristics |
gender: M
tissue: gastric
|
| Growth protocol |
Tumor tissues were collected after surgical resection and frozed for DNA extraction
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted using Gentra Puregene Cell Kit (Qiagen) following manufacturer’s instructions. QC process was performed using 1% agarose gel at 120V for 45min and single clear band with its size ~4000bp was defined as QC pass.
|
| Label |
Cy3
|
| Label protocol |
2 µg of genomic DNA were primed with 5µl random DNA primer at 96°C for 5 min
|
| |
|
| |
| Hybridization protocol |
Oligoarray control targets and hybridization buffer were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequential
|
| Scan protocol |
Scanned on an Agilent scanner.
Images were quantified using Agilent Feature Extraction Software (v 9.1)
|
| Data processing |
Agilent Feature Extraction Software (v 9.1) was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
May 05, 2014 |
| Last update date |
May 06, 2014 |
| Contact name |
Hua Dong |
| E-mail(s) |
hdong.az@gmail.com
|
| Organization name |
astrazeneca
|
| Street address |
199 Liangjing Rd.
|
| City |
Shanghai |
| ZIP/Postal code |
200013 |
| Country |
China |
| |
|
| Platform ID |
GPL4091 |
| Series (2) |
| GSE57302 |
Frozen tumour samples of 131 gastric cancer patients [array CGH] |
| GSE57308 |
Array CGH and expression data from gastric cancer patients |
|
