|
| Status |
Public on Jul 22, 2014 |
| Title |
GW-2 |
| Sample type |
RNA |
| |
|
| Source name |
GW4064 treated WT mouse 5
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
genotype/variation: wild type
tissue: liver
developmental stage: adult
|
| Treatment protocol |
C57BL/6J mice were treated with GW4064 at 100mg per kg body weight or vehicle control. Mice were treated three times, first dose at 8 am, second dose at 6 pm, third dose at 8 am the second day. Mice were sacrificed 2 hours after the last dose, and liver tissues were harvested for analysis.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from livers was prepared with TRIzol Reagent (Invitrogen, CA)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridizedon GeneChip Mouse Gene 1.0 ST Array with standard protocol.
|
| Scan protocol |
Not provided.
|
| Description |
gene expression data from GW4064 treated WT mouse liver
|
| Data processing |
The cel files were analyzed with Expression Cole 1.3.1 from Affymetrix using default analysis settings.
|
| |
|
| Submission date |
May 05, 2014 |
| Last update date |
Jul 22, 2014 |
| Contact name |
Le Zhan |
| E-mail(s) |
zhanle999@gmail.com
|
| Phone |
9135530042
|
| Organization name |
Rutgers University
|
| Department |
Pharmacology and Toxicology
|
| Lab |
EOHSI 332
|
| Street address |
170 Frelinghuysen Rd
|
| City |
Piscataway |
| State/province |
NJ |
| ZIP/Postal code |
08854 |
| Country |
USA |
| |
|
| Platform ID |
GPL8321 |
| Series (2) |
| GSE57305 |
Altered mRNA expression profile of GW4064 treated mouse livers compared to vehicle control |
| GSE57312 |
ChIP-seq and RNA-seq study of human FXR in primary human hepatocytes and microarray analysis of GW4064 versus vehicle treated wild type mouse livers |
|
