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Status |
Public on Jan 20, 2015 |
Title |
PatientID: 31269 |
Sample type |
RNA |
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Source name |
MM patient, previously treated, CD138+ selected cells
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Organism |
Homo sapiens |
Characteristics |
cell type: CD138+ selected bone marrow plasma cells disease status: multiple myeloma treatment status: previously treated treatment: TT6 molecular subgroup: HY gep70 score: -0.9493 OS time: 371 os censored: 0
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Extracted molecule |
total RNA |
Extraction protocol |
Following Ficoll-Hypaque gradient centrifugation, plasma cells obtained from the bone marrow were isolated from the mononuclear cell fraction by immunomagnetic bead selection using a monoclonal mouse anti-human CD138 antibody (Miltenyi-Biotec, Auburn, CA). More than 90 percent of the cells used for gene expression profiling were plasma cells, as shown by two-color flow cytometry using CD138+/CD45- and CD38+/CD45- markers, the presence of cytoplasmic immunoglobulin light chains by immunocytochemistry, and morphology by Wright-Giemsa staining. RNA was isolated with RNeasy Mini Kit (Qiagen, Valencia, CA).
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Label |
biotin
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Label protocol |
Samples were labels using the Affymetrix 3' IVT Express kit, according to the manufacturer's instructions.
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Hybridization protocol |
Samples were hybridized, washed and stained using the Affymetrix GeneChip Hybridization, Wash and Stain kit (P/N 900720) according to the manufacturer's recommendations.
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Scan protocol |
Chips were scanned using the HG-U133 Plus 2.0 v2.2 on an Affymetrix Autoloader 3000DX scanner.
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Data processing |
Data are processed using the 'affy' Bioconductor library packages for R.
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Submission date |
May 05, 2014 |
Last update date |
Jan 20, 2015 |
Contact name |
Christoph J Heuck |
E-mail(s) |
cjheuck@uams.edu
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Organization name |
University of Arkansas for Medical Sciences
|
Department |
Myeloma Institute for Research and Therapy
|
Street address |
4301 W Markham
|
City |
Little Rock |
State/province |
AR |
ZIP/Postal code |
72205 |
Country |
USA |
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|
Platform ID |
GPL570 |
Series (1) |
GSE57317 |
Gene expression profiles of patients with multiple myeloma who have been treated previously |
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