|
| Status |
Public on Jan 20, 2015 |
| Title |
PatientID: 31994 |
| Sample type |
RNA |
| |
|
| Source name |
MM patient, previously treated, CD138+ selected cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: CD138+ selected bone marrow plasma cells
disease status: multiple myeloma
treatment status: previously treated
treatment: TT6
molecular subgroup: CD-2
gep70 score: 0.1946
OS time: 217
os censored: 0
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Following Ficoll-Hypaque gradient centrifugation, plasma cells obtained from the bone marrow were isolated from the mononuclear cell fraction by immunomagnetic bead selection using a monoclonal mouse anti-human CD138 antibody (Miltenyi-Biotec, Auburn, CA). More than 90 percent of the cells used for gene expression profiling were plasma cells, as shown by two-color flow cytometry using CD138+/CD45- and CD38+/CD45- markers, the presence of cytoplasmic immunoglobulin light chains by immunocytochemistry, and morphology by Wright-Giemsa staining. RNA was isolated with RNeasy Mini Kit (Qiagen, Valencia, CA).
|
| Label |
biotin
|
| Label protocol |
Samples were labels using the Affymetrix 3' IVT Express kit, according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Samples were hybridized, washed and stained using the Affymetrix GeneChip Hybridization, Wash and Stain kit (P/N 900720) according to the manufacturer's recommendations.
|
| Scan protocol |
Chips were scanned using the HG-U133 Plus 2.0 v2.2 on an Affymetrix Autoloader 3000DX scanner.
|
| Data processing |
Data are processed using the 'affy' Bioconductor library packages for R.
|
| |
|
| Submission date |
May 05, 2014 |
| Last update date |
Jan 20, 2015 |
| Contact name |
Christoph J Heuck |
| E-mail(s) |
cjheuck@uams.edu
|
| Organization name |
University of Arkansas for Medical Sciences
|
| Department |
Myeloma Institute for Research and Therapy
|
| Street address |
4301 W Markham
|
| City |
Little Rock |
| State/province |
AR |
| ZIP/Postal code |
72205 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE57317 |
Gene expression profiles of patients with multiple myeloma who have been treated previously |
|
