|
| Status |
Public on Jul 30, 2014 |
| Title |
Sle1.3/het #1 |
| Sample type |
RNA |
| |
|
| Source name |
splenocytes from sle1.3/E2-2het mice
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57/B6
genotype/variation: sle1.3/E2-2het; Sle1.3 mice haplodeficient for E2-2
gender: male
tissue: spleen
cell type: splenocytes
|
| Treatment protocol |
Collagenase/DnaseI digestion of the spleen
|
| Growth protocol |
none
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from Trizol LS according to manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
We used the Ambion WT Labelling Kit as per manufacturer's instructions. We used 250 ng/sample of starting total RNA.
|
| |
|
| Hybridization protocol |
Biotinylated DNA probe was made according to the standard Affymetrix protocol.
|
| Scan protocol |
Arrays were scanned according to the Affymetrix protocol.
|
| Description |
Spleen Sle1.3/E2-2het #1; Sle1.3/E2-2het #1
|
| Data processing |
Gene level RMA using Affymetrix expression console software
|
| |
|
| Submission date |
May 06, 2014 |
| Last update date |
Jul 30, 2014 |
| Contact name |
Vanjs Sisirak |
| E-mail(s) |
vs2376@columbia.edu
|
| Phone |
212 342 2936
|
| Organization name |
Columbia University
|
| Department |
Microbiology and Immunology
|
| Lab |
Reizis Lab
|
| Street address |
701 west 168th street
|
| City |
New York City |
| State/province |
NY |
| ZIP/Postal code |
10033 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE57324 |
Gene expression analysis in the spleen of wild type, Sle1.3 (lupus mice) and Sle1.3 mice haplodeficient for E2-2 (exhibiting non functional pDCs) |
|
