|
| Status |
Public on May 07, 2014 |
| Title |
VEC Bio Rep1 Tech rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Mammary epithelial Cell Line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF10A
expression: Control vector
treatment: without ERBB4 ligand NRG1 for 2h
|
| Treatment protocol |
Control vector (VEC) and ERBB4 expression MCF10A cells(CYT1 or CYT2) were serum starved for 48 hours and incubated with or without ERBB4 ligand NRG1 for 2h. RNA samples were collected and analyzed.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Plus Mini Kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Illumina TotalPrep RNA Amplification Kit.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Tech Replicate2
|
| Data processing |
Illumina Beadchip Studio, log2 transformed
|
| |
|
| Submission date |
May 06, 2014 |
| Last update date |
May 07, 2014 |
| Contact name |
James T. Platt |
| Organization name |
Yale University
|
| Department |
Medical Oncology
|
| Street address |
333 Cedar ST
|
| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06520 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE57346 |
Gene Expression Profiling on Stably Expressing Full Length (FL) JM-a CYT-1 or CYT-2 ERBB4 isoforms in MCF10A cells |
|
