|
| Status |
Public on Feb 11, 2015 |
| Title |
Wilms tissue 3 |
| Sample type |
RNA |
| |
|
| Source name |
Wilms tissue
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Wilms tissue
histological subtype: blastema
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA including miRNAs was isolated using theQiagen miRNeasy Kit according to manufacturers recommendations. RNA concentration was measured using NanoDrop2000 (ThermoScientific). RNA integrity was measured using the PicoRNA kit (Agilent) on a Bioanalyzer2100.
|
| Label |
Cyanine 3
|
| Label protocol |
The expression profile of 1205 human miRNAs was determined using SurePrint G3 8x60k miRNA microarrays (Agilent) according to the manufacturers recommendations, using miRNA Complete Labeling and Hyb Kit (Agilent, Cat. No 5190-0456), Cyanine 3-pcp
|
| |
|
| Hybridization protocol |
The expression profile of 1205 human miRNAs was determined using SurePrint G3 8x60k miRNA microarrays (Agilent) according to the manufacturers recommendations, using miRNA Complete Labeling and Hyb Kit (Agilent, Cat. No 5190-0456), Cyanine 3-pcp
|
| Scan protocol |
Arrays were scanned with the Agilent Scanner with default settings.
|
| Description |
total RNA including miRNA
miRNA expression in tumor
WS914TA4
|
| Data processing |
Raw expression values were extracted using the Feature Extraction Software of Agilent. Raw signal intensities were quantil-normalized.
|
| |
|
| Submission date |
May 07, 2014 |
| Last update date |
Feb 12, 2015 |
| Contact name |
Nicole Ludwig |
| E-mail(s) |
n.ludwig@mx.uni-saarland.de
|
| Phone |
004968411626269
|
| Organization name |
Saarland University
|
| Department |
Human Genetics
|
| Street address |
Kirrberger Strasse
|
| City |
Homburg |
| ZIP/Postal code |
66421 |
| Country |
Germany |
| |
|
| Platform ID |
GPL16770 |
| Series (1) |
| GSE57370 |
miRNA expression profiles in Wilms tumor subtypes |
|
