|
| Status |
Public on Apr 18, 2007 |
| Title |
C57BL/6J_female_hypothalamus |
| Sample type |
RNA |
| |
|
| Source name |
C57BL/6J_female_hypothalamus
|
| Organism |
Mus musculus |
| Characteristics |
C57BL/6J_female_hypothalamus
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Hypothalamus tissue was harvested from 32 strains of mice from the Mouse Diversity Panel. Total RNA was isolated from frozen tissue using Trizol, and cleaned up using an RNeasy kit (Qiagen). For the male samples, 1 ug total RNA from three separate samples was pooled and 3 ug used for cDNA synthesis and amplification. For the female samples, 1 ug total RNA from the hypothalamus of an individual mouse was used for cDNA synthesis and amplification.
|
| Label |
biotin
|
| Label protocol |
cDNA synthesis and amplification employed the Affymetrix 3' IVT protocol.
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures using the GeneChip Fluidics Station 450
|
| Scan protocol |
standard Affymetrix procedures using the GeneChip 3000 scanner
|
| Description |
C57BL/6J_female_hypothalamus
|
| Data processing |
MAS5
|
| |
|
| Submission date |
Oct 03, 2006 |
| Last update date |
Apr 18, 2007 |
| Contact name |
John R Walker |
| E-mail(s) |
jwalker@gnf.org
|
| Phone |
858-812-1636
|
| Organization name |
Genomics Institute of the Novartis Research Foundation
|
| Lab |
Genetics Core
|
| Street address |
10675 John Jay Hopkins
|
| City |
San Diego |
| State/province |
CA |
| ZIP/Postal code |
92121 |
| Country |
USA |
| |
|
| Platform ID |
GPL1073 |
| Series (1) |
| GSE5961 |
Genome-wide association analysis in diverse inbred mice: power and population structure |
|
