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Sample GSM1387742 Query DataSets for GSM1387742
Status Public on Dec 24, 2015
Title PBMC from patient showing IFTA with inflammation in renal biopsy:i.IFTA_1
Sample type RNA
 
Source name PBMC from patients with signs of tubular atrophy and interstitial fibrosis (IFTA)
Organism Homo sapiens
Characteristics renal biopsy group: signs of tubular atrophy and interstitial fibrosis (IFTA)
cell type: Peripheral blood mononuclear cells (PBMC)
Extracted molecule total RNA
Extraction protocol For total RNA extraction cryopreserved liver tissue samples were homogenized in TRIzol reagent (Invitrogen, San Diego, CA, USA) using pestle and nuclease-free 1,5ml reaction tubes (Ambion). Total RNA was then extracted following the manufacturers guidelines and quality was assessed with the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, USA).
Label biotin
Label protocol Standard Affymetrix protocol
 
Hybridization protocol Standard Affymetrix protocol
Scan protocol Standard Affymetrix protocol using a Gene chip scanner 3000
Description i.IFTA_1
Data processing Data processing cel files were normalized with 'rma' function of the Bioconductor package (v2.13) 'rma' in R.3.0.1
 
Submission date May 16, 2014
Last update date Dec 24, 2015
Contact name Juanjo Lozano
E-mail(s) juanjo.lozano@ciberehd.org
Organization name CIBEREHD
Department Plataforma de Bioinformatica
Street address C/ Rosselló 153
City Barcelona
ZIP/Postal code 08036
Country Spain
 
Platform ID GPL17244
Series (1)
GSE57731 Blood gene expression at 3 months post-transplantation predicts 1-year histologic features in renal transplantation

Data table header descriptions
ID_REF
VALUE log2 rma signal intensity

Data table
ID_REF VALUE
100009676_at 8.560611751
10000_at 9.058887634
10001_at 9.480298335
10002_at 6.466777933
100033413_at 4.258232625
100033414_at 7.162886064
100033416_at 4.685741641
100033418_at 4.663896246
100033420_at 4.378879793
100033422_at 3.867840748
100033423_at 4.411827017
100033424_at 3.896036639
100033425_at 3.683176946
100033426_at 6.313447024
100033427_at 7.503536805
100033428_at 3.953744548
100033430_at 3.546966851
100033431_at 5.314071246
100033432_at 5.207175351
100033433_at 3.737574857

Total number of rows: 19684

Table truncated, full table size 400 Kbytes.




Supplementary file Size Download File type/resource
GSM1387742_i.IFTA_1.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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