|
| Status |
Public on Dec 24, 2015 |
| Title |
PBMC from patient showing IFTA with inflammation in renal biopsy:i.IFTA_8 |
| Sample type |
RNA |
| |
|
| Source name |
PBMC from patients with IFTA with inflammation
|
| Organism |
Homo sapiens |
| Characteristics |
renal biopsy group: IFTA with inflammation (i-IFTA)
cell type: Peripheral blood mononuclear cells (PBMC)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
For total RNA extraction cryopreserved liver tissue samples were homogenized in TRIzol reagent (Invitrogen, San Diego, CA, USA) using pestle and nuclease-free 1,5ml reaction tubes (Ambion). Total RNA was then extracted following the manufacturers guidelines and quality was assessed with the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, USA).
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol
|
| Scan protocol |
Standard Affymetrix protocol using a Gene chip scanner 3000
|
| Description |
i.IFTA_8
|
| Data processing |
Data processing cel files were normalized with 'rma' function of the Bioconductor package (v2.13) 'rma' in R.3.0.1
|
| |
|
| Submission date |
May 16, 2014 |
| Last update date |
Dec 24, 2015 |
| Contact name |
Juanjo Lozano |
| E-mail(s) |
juanjo.lozano@ciberehd.org
|
| Organization name |
CIBEREHD
|
| Department |
Plataforma de Bioinformatica
|
| Street address |
C/ Rosselló 153
|
| City |
Barcelona |
| ZIP/Postal code |
08036 |
| Country |
Spain |
| |
|
| Platform ID |
GPL17244 |
| Series (1) |
| GSE57731 |
Blood gene expression at 3 months post-transplantation predicts 1-year histologic features in renal transplantation |
|
