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Sample GSM1387753 Query DataSets for GSM1387753
Status Public on Dec 24, 2015
Title PBMC from patient showing IFTA with inflammation in renal biopsy:i.IFTA_12
Sample type RNA
 
Source name PBMC from patients with IFTA with inflammation
Organism Homo sapiens
Characteristics renal biopsy group: IFTA with inflammation (i-IFTA)
cell type: Peripheral blood mononuclear cells (PBMC)
Extracted molecule total RNA
Extraction protocol For total RNA extraction cryopreserved liver tissue samples were homogenized in TRIzol reagent (Invitrogen, San Diego, CA, USA) using pestle and nuclease-free 1,5ml reaction tubes (Ambion). Total RNA was then extracted following the manufacturers guidelines and quality was assessed with the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, USA).
Label biotin
Label protocol Standard Affymetrix protocol
 
Hybridization protocol Standard Affymetrix protocol
Scan protocol Standard Affymetrix protocol using a Gene chip scanner 3000
Description i.IFTA_12
Data processing Data processing cel files were normalized with 'rma' function of the Bioconductor package (v2.13) 'rma' in R.3.0.1
 
Submission date May 16, 2014
Last update date Dec 24, 2015
Contact name Juanjo Lozano
E-mail(s) juanjo.lozano@ciberehd.org
Organization name CIBEREHD
Department Plataforma de Bioinformatica
Street address C/ Rosselló 153
City Barcelona
ZIP/Postal code 08036
Country Spain
 
Platform ID GPL17244
Series (1)
GSE57731 Blood gene expression at 3 months post-transplantation predicts 1-year histologic features in renal transplantation

Data table header descriptions
ID_REF
VALUE log2 rma signal intensity

Data table
ID_REF VALUE
100009676_at 8.498978179
10000_at 9.590746934
10001_at 9.813126123
10002_at 6.637762922
100033413_at 4.525183233
100033414_at 7.625090341
100033416_at 5.332309726
100033418_at 5.171272833
100033420_at 4.862743736
100033422_at 3.878255595
100033423_at 4.459760447
100033424_at 3.828865621
100033425_at 3.80534641
100033426_at 7.192007772
100033427_at 7.705049724
100033428_at 3.924978029
100033430_at 3.573862206
100033431_at 5.458903062
100033432_at 5.039789934
100033433_at 3.752520716

Total number of rows: 19684

Table truncated, full table size 400 Kbytes.




Supplementary file Size Download File type/resource
GSM1387753_i.IFTA_12.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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