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Status |
Public on Dec 24, 2015 |
Title |
PBMC from patient showing IFTA with inflammation in renal biopsy:i.IFTA_12 |
Sample type |
RNA |
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|
Source name |
PBMC from patients with IFTA with inflammation
|
Organism |
Homo sapiens |
Characteristics |
renal biopsy group: IFTA with inflammation (i-IFTA) cell type: Peripheral blood mononuclear cells (PBMC)
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Extracted molecule |
total RNA |
Extraction protocol |
For total RNA extraction cryopreserved liver tissue samples were homogenized in TRIzol reagent (Invitrogen, San Diego, CA, USA) using pestle and nuclease-free 1,5ml reaction tubes (Ambion). Total RNA was then extracted following the manufacturers guidelines and quality was assessed with the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, USA).
|
Label |
biotin
|
Label protocol |
Standard Affymetrix protocol
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|
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Hybridization protocol |
Standard Affymetrix protocol
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Scan protocol |
Standard Affymetrix protocol using a Gene chip scanner 3000
|
Description |
i.IFTA_12
|
Data processing |
Data processing cel files were normalized with 'rma' function of the Bioconductor package (v2.13) 'rma' in R.3.0.1
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Submission date |
May 16, 2014 |
Last update date |
Dec 24, 2015 |
Contact name |
Juanjo Lozano |
E-mail(s) |
juanjo.lozano@ciberehd.org
|
Organization name |
CIBEREHD
|
Department |
Plataforma de Bioinformatica
|
Street address |
C/ Rosselló 153
|
City |
Barcelona |
ZIP/Postal code |
08036 |
Country |
Spain |
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|
Platform ID |
GPL17244 |
Series (1) |
GSE57731 |
Blood gene expression at 3 months post-transplantation predicts 1-year histologic features in renal transplantation |
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