|
| Status |
Public on Sep 01, 2017 |
| Title |
P11_T3_VALIDATION |
| Sample type |
RNA |
| |
|
| Source name |
female patient, no treatment
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
|
| Treatment protocol |
Patients undergoing heart surgery on CPB were recruited in a prospective, multicenter, randomized, double-blind study. In brief, RIPC was induced during standardized anesthesia by four 5-min cycles of upper limb ischemia and 5-min reperfusion using a blood-pressure cuff inflated to a pressure 200 mm Hg, whereas the pressure was at least 20 mm Hg greater than the systolic arterial pressure measured via the arterial line. The study intervention was performed by un-blinded staff either on the patient’s arm (RIPC) or on a standardized dummy arm (control). The 12 Patients were approx. 60 years old (mean: RIPC 61.2, CTRL 57.6; sd: RIPC 10.9, CTRL 11.2).
|
| Growth protocol |
All samples were taken after official registration (ClinicalTrials.gov Identifier: NCT01067703) and written consent.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Blood was drawn before CPB (T1), 30 min after CPB (T2) and at 8:00 on the first postoperative day (T3) and prepared with 200 μl PAXgene reagent (PreAnalytix®, Qiagen, Hilden, Germany). Biotinylated probes were prepared from 500 ng of total RNA, using the TargetAmp™-Nano Labeling Kit for Illumina® Expression BeadChip®. Amplification of RNA was in agreement to the protocol of the Illumina® for the BeadArray Reader 500 X.
|
| Label |
biotin
|
| Label protocol |
Amplification and biotinylation of RNA were carried out using the TargetAmp™-Nano Labeling Kit for Illumina® Expression BeadChip®.
|
| |
|
| Hybridization protocol |
Biotinylated probes were prepared from 500 ng of total RNA, using the TargetAmp™-Nano Labeling Kit for Illumina® Expression BeadChip®. Amplification of RNA was in agreement to the protocol of the Illumina® for the BeadArray Reader 500 X.
|
| Scan protocol |
Arrays were readout with the detection system iScan from Illumina.
|
| Description |
in vivo
|
| Data processing |
Raw data was pre-processed using GenomeStudio (v 1.9.0). Robust spline normalization (rsn) and detection filtering were performed via the lumi package within Bioconductor and R statistics software (version 3.03). Detection (p<0.01) was required in at least four samples from the 34 initial samples. Ten further samples were taken for validation (techn. replicates). Annotation was mapped using HumanHT-12_V4_0_R2_15002873_B from Illumina.
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| |
|
| Submission date |
Jun 06, 2014 |
| Last update date |
Sep 01, 2017 |
| Contact name |
S. Lambeck |
| Organization name |
JUH
|
| Street address |
Erlanger Allee 101
|
| City |
Jena |
| ZIP/Postal code |
07743 |
| Country |
Germany |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE58270 |
Transcriptome profiling of remote ischaemic preconditioning effects for heart surgery |
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