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Status |
Public on Oct 21, 2014 |
Title |
Control1-DHT |
Sample type |
RNA |
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|
Source name |
prostate cancer
|
Organism |
Homo sapiens |
Characteristics |
cell line: LNCaP knockdown: siControl treatment: DHT 10 nM
|
Treatment protocol |
Cells were transfected with each siRNA 48 h before hormone treatment. Cells were treated with vehicle or DHT (10 nM).
|
Growth protocol |
LNCaP cells were cultures in RPMI 1640 containing 10% FBS. Before hormone treatment, cells were incubated in Phenol red-free RPMI 1640 containing 2.5% charcoal/dextran treated FBS for three days.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNAs were extracted using ISOGEN (Nippon gene) according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Total RNA was labeled employing the Affymetrix GeneChip Whole Transcript (WT) Terminal Labeling kit according to the manufacturer’s instructions
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|
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Hybridization protocol |
The samples were hybridized to Human Gene 1.0 ST microarrays according to standardized protocols from Affymetrix.
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Scan protocol |
The samples were scanned on a GeneChip Scanner 3000 7G (Affymetrix).
|
Description |
Gene expression data from LNCaP cells treated with DHT 10 nM after transfected with siControl
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Data processing |
The microarray data were preprocessed with ver 1.1.1 of Affymetrix Expression Console software using Affymetrix default Robust Multichip Analysis (RMA) sketch algorithm workflow.
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Submission date |
Jun 09, 2014 |
Last update date |
Oct 21, 2014 |
Contact name |
Ken-ichi Takayama |
Organization name |
Tokyo Metropolitan Institute of Gerontology
|
Street address |
Sakaecho
|
City |
Itabashi-ku |
ZIP/Postal code |
173-0015 |
Country |
Japan |
|
|
Platform ID |
GPL6244 |
Series (1) |
GSE58309 |
The role of AR-associated factors in androgen signaling |
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