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Sample GSM1406292 Query DataSets for GSM1406292
Status Public on Oct 21, 2014
Title Contorl2-Et
Sample type RNA
 
Source name prostate cancer
Organism Homo sapiens
Characteristics cell line: LNCaP
knockdown: siControl
treatment: Vehicle
Treatment protocol Cells were transfected with each siRNA 48 h before hormone treatment. Cells were treated with vehicle or DHT (10 nM).
Growth protocol LNCaP cells were cultures in RPMI 1640 containing 10% FBS. Before hormone treatment, cells were incubated in Phenol red-free RPMI 1640 containing 2.5% charcoal/dextran treated FBS for three days.
Extracted molecule total RNA
Extraction protocol Total RNAs were extracted using ISOGEN (Nippon gene) according to the manufacturer's instructions.
Label biotin
Label protocol Total RNA was labeled employing the Affymetrix GeneChip Whole Transcript (WT) Terminal Labeling kit according to the manufacturer’s instructions
 
Hybridization protocol The samples were hybridized to Human Gene 1.0 ST microarrays according to standardized protocols from Affymetrix.
Scan protocol The samples were scanned on a GeneChip Scanner 3000 7G (Affymetrix).
Description Gene expression data from LNCaP cells treated with vehicle (ethanol) after transfected with siControl
Data processing The microarray data were preprocessed with ver 1.1.1 of Affymetrix Expression Console software using Affymetrix default Robust Multichip Analysis (RMA) sketch algorithm workflow.
 
Submission date Jun 09, 2014
Last update date Oct 21, 2014
Contact name Ken-ichi Takayama
Organization name Tokyo Metropolitan Institute of Gerontology
Street address Sakaecho
City Itabashi-ku
ZIP/Postal code 173-0015
Country Japan
 
Platform ID GPL6244
Series (1)
GSE58309 The role of AR-associated factors in androgen signaling

Data table header descriptions
ID_REF
VALUE log2 RMA signal

Data table
ID_REF VALUE
7892501 6.9991
7892502 5.31255
7892503 3.69158
7892504 8.89541
7892505 3.46439
7892506 4.63528
7892507 6.43935
7892508 8.12443
7892509 11.8008
7892510 4.97207
7892511 4.96719
7892512 7.93925
7892513 4.33036
7892514 11.185
7892515 9.32099
7892516 6.59535
7892517 8.05339
7892518 3.26876
7892519 4.53784
7892520 9.16796

Total number of rows: 33297

Table truncated, full table size 516 Kbytes.




Supplementary file Size Download File type/resource
GSM1406292_9-Control2-Et_HuGene-1_0-st-v1_.CEL.gz 4.2 Mb (ftp)(http) CEL
GSM1406292_9-Control2-Et_HuGene-1_0-st-v1_.rma-gene-default.chp.gz 255.7 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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