|
| Status |
Public on Jul 01, 2014 |
| Title |
human fetal alpha 6 integrin-CD133- epidermal population sort 3356 |
| Sample type |
RNA |
| |
|
| Source name |
epidermis from 13 week human fetal scalp
|
| Organism |
Homo sapiens |
| Characteristics |
age: 13 weeks
tissue: fetal scalp epidermis
tissue population: alpha6integrin-/CD133- epidermal population
|
| Treatment protocol |
Tissue was dispase-treated to isolate epidermis.Single epidermal cells, obtained by rapid trypsin treatment and mechanical dissociation, were sorted for expression of alpha-6 integrin and CD133 and RNA extracted for gene array analyses.
|
| Growth protocol |
13 week human fetal scalp tissue was obtained from 1st trimester abortions in conformity with federal and state laws and following National Institute of Health Human Fetal Tissue Research Review Panel of 1988 guidelines. 13 week gestation was defined based upon observance of hair follicle placodes and buds but not external hair shafts.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the Qiagen Rneasy Plus kit
|
| Label |
biotin
|
| Label protocol |
cRNA, amplified from 250ng total RNA, was converted to cDNA, fragmented, assessed by Bioanalyzer, and biotinylated by terminal transferase end labeling
|
| |
|
| Hybridization protocol |
5.5ug of labeled cDNA was added to Affymetrix hybridization cocktails, heated at 99ºC for 5 min and hybridized for 16 h at 45ºC to Human Gene 1.0ST GeneChips (Affymetrix Inc., Santa Clara CA)
|
| Scan protocol |
Gene chips were scanned using a GeneChip 3000 7G scanner.
|
| Description |
Gene expression data from sort 3356 human fetal scalp alpha 6 integrin-CD133- epidermal cells.
3356_DN
|
| Data processing |
CEL files were normalized with RMA in Partek Genomics Suite v6.6
|
| |
|
| Submission date |
Jun 11, 2014 |
| Last update date |
Jul 01, 2014 |
| Contact name |
Denise Gay |
| E-mail(s) |
denise.gay@nyulangone.org
|
| Organization name |
NYU langone
|
| Street address |
550 1st avenue
|
| City |
New York |
| ZIP/Postal code |
10016 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE58393 |
Expression data from 13 week human fetal scalp epidermis sorted for expression of alpha 6 integrin and CD133 |
|
