|
| Status |
Public on Nov 30, 2006 |
| Title |
Rap1-Tap IP during post-diauxic shift (72 hrs) culture #7 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Rap1-Tap IP
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
Rap1-Tap IP during post-diauxic shift (72 hrs) culture #7
|
| Treatment protocol |
Rich Media (YPD 2% Glucose)
|
| Growth protocol |
Post-Diauxic Shift 72 hrs
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
IP DNA isoloated from 1mg protein from WCE by affinity purification of Rap1-Tap. Using Tap-Tag TEV cleavage protocol.
|
| Label |
Cy3
|
| Label protocol |
samples and references were amplified using a random primed PCR-based method (see Bohlander et al., 1992)
|
| |
|
| Channel 2 |
| Source name |
Rap1-Tap Ref
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
Rap1-Tap Input Reference
|
| Treatment protocol |
Rich Media (YPD 2% Glucose)
|
| Growth protocol |
Post-Diauxic Shift 72 hrs
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Input genomic DNA, 1/20 of WCE for IP used as input. Reversed and crosslinked, purified, and amplified using Rnd A/B/C.
|
| Label |
Cy5
|
| Label protocol |
samples and references were amplified using a random primed PCR-based method (see Bohlander et al., 1992)
|
| |
|
| |
| Hybridization protocol |
Direct hybridization 4X SSC, 0.2% SDS
|
| Scan protocol |
Images were acquired using a GenePix 4000B scanner and Genepix software (Axon Instruments)
|
| Description |
Rap1 ChIP-chip using Tap affinity tag on culture # 7 during post-diuaxic shift (72 hrs) OD 4.960 in the absence of glucose. Dye-swap Strain - Rap1-Tap, BY4741
|
| Data processing |
The normalized median log2 ratios (ch2/ch1) were download from UNC microarray database, dye swapped experiments were inverted
|
| |
|
| Submission date |
Oct 22, 2006 |
| Last update date |
Jan 23, 2007 |
| Contact name |
Jason D. Lieb |
| E-mail(s) |
jlieb@bio.unc.edu
|
| Phone |
919-843-3228
|
| Organization name |
Univeristy of North Carolina at Chapel Hill
|
| Department |
Biology
|
| Lab |
Lieb
|
| Street address |
CB 3280
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27599 |
| Country |
USA |
| |
|
| Platform ID |
GPL4414 |
| Series (1) |
| GSE5948 |
A chromatin-mediated mechanism for specification of conditional transcription factor targets |
|
