|
| Status |
Public on Feb 18, 2007 |
| Title |
SAGE of 9-DPA cotton fiber |
| Sample type |
SAGE |
| Anchor |
NlaIII
|
| Tag Count |
57314 |
| Tag Length |
10 |
| |
|
| Source name |
Cotton fiber at 9 DPA
|
| Organism |
Gossypium hirsutum |
| Characteristics |
9-DPA cotton fiber
|
| Treatment protocol |
No treatment.
|
| Growth protocol |
Plants of Upland Cotton, Gossypium hirsutum L. cv. Xu-142 were grown in field. Bolls were tagged and the flowering day was taken as 0 day post-anthesis (DPA). Ovules at 9 DPA were collected in the morning, and the material was frozen in liquid nitrogen and stored at -70 C before use.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA for SAGE library construction was isolated from the 9 DPA fibers by a cold-phenol method as previously described (Li et al. 2002).
|
| |
|
| Description |
30 μg total RNA from 9 DPA Gossypium. hirsutum Xu-142 c.v. fiber was used to construct the SAGE library by the I-SAGE kit (Invitrogen, Carlsbad, CA) according to the manual.
|
| Data processing |
The software SAGE2000 ver4.5 (courtesy of Ken Kinzler and Victor Velculescu, Johns Hopkins University School of Medicine) was applied to analyze the sequence data.
|
| |
|
| Submission date |
Oct 23, 2006 |
| Last update date |
Nov 19, 2008 |
| Contact name |
Xiao-Ya Chen |
| E-mail(s) |
xychen@sibs.sc.cn
|
| Phone |
86-21-54924033
|
| Fax |
86-21-54924015
|
| URL |
http://xy.sippe.ac.cn/
|
| Organization name |
Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
|
| Department |
Institute of Plant Physiology and Ecology
|
| Lab |
National Key Laboratory of Plant Molecular Genetics
|
| Street address |
No. 300, Fenglin Road
|
| City |
Shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
| |
|
| Platform ID |
GPL4474 |
| Series (1) |
| GSE6103 |
SAGE of cotton fibers and ovules. |
|
