|
| Status |
Public on Jun 21, 2014 |
| Title |
EV1 |
| Sample type |
RNA |
| |
|
| Source name |
3T3 vector
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL6J
transduction: vector
cell type: Mouse Embryonic Fibroblasts
cell line: 3T3
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Qiagen Rneasy Mini Prep kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol-Direct Hybridization
|
| Scan protocol |
Standard Illumina scanning protocol-Illumina iScan System
|
| Description |
Replicate A
Illumina® TotalPrep RNA Amplification Kit, manufactured by Ambion, Inc.
|
| Data processing |
The data were normalized using quantile normalization using genome studio software
|
| |
|
| Submission date |
Jun 20, 2014 |
| Last update date |
Jun 21, 2014 |
| Contact name |
Po-Yuan Tung |
| E-mail(s) |
ptung@ucdavis.edu
|
| Organization name |
UC Davis
|
| Lab |
Paul Knoepfler
|
| Street address |
2425 Stockton Blvd
|
| City |
Sacramento |
| State/province |
CA |
| ZIP/Postal code |
95817 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE58709 |
Total Gene expression analysis of Dppa4-trandformed 3T3s |
|
