|
| Status |
Public on Jun 21, 2014 |
| Title |
prostate_Klf5 ko_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
6-month mouse dorsal prostate
|
| Organism |
Mus musculus |
| Characteristics |
strain: B6/129
gender: male
age: 6 months
tissue: dorsal prostate
genotype: Klf5-/-; Pten-/-;PB-Cre4
|
| Treatment protocol |
No any treatment, normally maintained
|
| Growth protocol |
mouse in the same generation maintained in the same SPF animal facility of Emory University following IACUC protocol
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the RNeasy Mini Kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
RNA was biotin labeled at the Emory Integrated Genomics Core at Emory University following manufacturers instructions.
|
| |
|
| Hybridization protocol |
Labeled, fragmented RNA hybridized to Affymetrix Mouse Gene 1.0 ST Gene Arrays at the Emory Integrated Genomics Core at Emory University following manufacturers instructions.
|
| Scan protocol |
Arrays were scanned at the Emory Integrated Genomics Core at Emory University following manufacturers instructions.
|
| Description |
Gene expression data from Klf5-KO 6-month mouse dorsal prostate with Pten-null upon PB-Cre4 mediated floxed fragment deletion
|
| Data processing |
The data was analyzed by the Emory Integrated Genomics Core at Emory University using mean polish and RMA with GC content adjustment.
|
| |
|
| Submission date |
Jun 21, 2014 |
| Last update date |
Jun 21, 2014 |
| Contact name |
Jin-Tang Dong |
| Organization name |
Emory University
|
| Department |
Department of Hematology & Medical Oncology
|
| Street address |
1365C Clifton Rd
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30322 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE58719 |
Expression data from conditional Klf5 knockout Pten-null mouse prostates |
|
