|
| Status |
Public on Nov 05, 2014 |
| Title |
EB |
| Sample type |
RNA |
| |
|
| Source name |
hESC
|
| Organism |
Homo sapiens |
| Characteristics |
esc cell line: EB
genotype/variation: MEG3-ON
neural differentiation capacity: not detected
|
| Growth protocol |
ReproCELL primate and human ES Cell culture medium on MEF;
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from the cells were isolated using TRIzol® reagent (Invitrogen, Calsbad, CA) and purified with RNeasy® mini kits (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
6247231044_E
|
| Data processing |
Quantile normalization were carried out using Partek commercial microarray analysis software
|
| |
|
| Submission date |
Jun 25, 2014 |
| Last update date |
Nov 05, 2014 |
| Contact name |
Shau-Ping Lin |
| E-mail(s) |
shaupinglin@ntu.edu.tw
|
| Organization name |
National Taiwan University
|
| Department |
Institute of Biotechnology
|
| Street address |
4F, No. 81, Chang-Xing St.,
|
| City |
Taipei City |
| State/province |
select a state |
| ZIP/Postal code |
106 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE58809 |
Loss of non-coding RNA expression from the DLK1-DIO3 imprinted locus correlates with reduced neural differentiation potential in human embryonic stem cell lines |
|
