|
| Status |
Public on Dec 31, 2014 |
| Title |
MCF7 hGH |
| Sample type |
RNA |
| |
|
| Source name |
MCF7 clone transfected with pMT-hGH
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF7
cell type: Breast cancer
transfection: pMT-hGH
|
| Treatment protocol |
MCF-MUT and MCF-hGH were cultured for a total of 24 h in serum-free medium before processing for RNA preparation.
|
| Growth protocol |
MCF7-MUT and MCF7-hGH were cultured at 37 °C in 5% CO2 in completed RPMI-1640 medium supplemented with 10% heat-inactivated fetal bovine serum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The total RNA and miRNA was isolated from cells by using the TRIzol Reagent (Life Technologies) and mirVana(TM) Isolation Kit (Invitrogen).
|
| Label |
Cy3
|
| Label protocol |
The total miRNA was labeled by useing the mirVana(TM) miRNA Labeling kit (Ambion) as per the manufacturer's protocol.
|
| |
|
| Hybridization protocol |
The labeled miRNA was hybridized as per the manufacturer's protocol of mirVana(TM) miRNA bioarray(Invitrogen).
|
| Scan protocol |
The hybridized slides were scanned on the microarray scanner LuxScan TM 10K(CapitalBio, Ltd) at 532nm and then were processed with GenePix Pro 6.0 (Axon, Ltd) and signal intensities quantified.
|
| Description |
pMT-hGH is an expression plasmid containing the wild-type hGH gene. This Sample represents 2 replicates.
|
| Data processing |
The mean of the duplicate detected intensities was taken and normalized to internal positive controls using Microsoft Excel.
|
| |
|
| Submission date |
Jun 26, 2014 |
| Last update date |
Dec 31, 2014 |
| Contact name |
Xiang H.-F. Zhang |
| Organization name |
Baylor College of Medicine
|
| Department |
Beast Center
|
| Street address |
One Baylor Plaza
|
| City |
Houston |
| State/province |
Texas |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL16593 |
| Series (1) |
| GSE58845 |
miRNA profiling of MCF7 autocrine hGH |
|
