|
| Status |
Public on Jun 26, 2017 |
| Title |
Δclp-1 |
| Sample type |
RNA |
| |
|
| Source name |
Lysobacter enzymogenes OH11 delta-clp mutant
|
| Organism |
Lysobacter enzymogenes |
| Characteristics |
strain: OH11
genotype: delta-clp mutant
|
| Growth protocol |
Lysobacter enzymogenes OH11 and delta-clp mutant were grown to mid-log phase (O.D.600nm 1.0) at 28°C in 10%TSB
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy Mini kit (Qiagen Inc., Valencia, CA, USA) and the RNAprotect reagent (Qiagen) and DNA was removed by on-column DNase digestion with the RNase-Free DNase set (Qiagen). RNA quality and concentration was determined by analysis with an Agilent 2100 bioanalyzer at the Harvard-Partners Center for Genetics and Genomics (Harvard Medical School, Cambridge, MA USA).
|
| Label |
Cy3
|
| Label protocol |
Labeling was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
|
| |
|
| Hybridization protocol |
Hybridization was performed by NimbleGen Systems Inc., Madison, WI, USA following their standard operating protocol. See www.nimblegen.com.
|
| Scan protocol |
Scanning was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
|
| Description |
This sample is of Lysobacter enzymogenes OH11 delta-clp mutant. It is the first of two mutant biological replicates used in this experiment, each from separate cultures.
|
| Data processing |
The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249), quantile normalization (Bolstad et al. Bioinformatics 19(2):185), and background correction as implemented in the NimbleScan software package, version 2.4.27 (Roche NimbleGen, Inc.).
|
| |
|
| Submission date |
Jun 26, 2014 |
| Last update date |
Jun 26, 2017 |
| Contact name |
Wang Yansheng |
| E-mail(s) |
wangyansheng@aliyun.com
|
| Organization name |
Nanjing Agricultural University
|
| Department |
Department of Plant Pathology
|
| Street address |
No.1 Weigang
|
| City |
Nanjing |
| ZIP/Postal code |
210095 |
| Country |
China |
| |
|
| Platform ID |
GPL18866 |
| Series (1) |
| GSE58846 |
Expression analysis of Lysobacter enzymogenes OH11 delta-clp mutant |
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