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Sample GSM142104 Query DataSets for GSM142104
Status Public on Nov 30, 2006
Title Rap1 IP with AB on nrg1del during expontential growth culture #2
Sample type genomic
 
Channel 1
Source name Ref for Nrg1del #2
Organism Saccharomyces cerevisiae
Characteristics Ref for Nrg1del #2
Treatment protocol Rich Media (YPD 2% Glucose)
Growth protocol Expontential Growth (OD 0.6-1.0)
Extracted molecule genomic DNA
Extraction protocol Input genomic DNA, 1/20 of WCE for IP used as input. Reversed and crosslinked, purified, and amplified using Rnd A/B/C.
Label Cy3
Label protocol samples and references were amplified using a random primed PCR-based method (see Bohlander et al., 1992)
 
Channel 2
Source name Rap1 IP on Nrg1del #2 during log phase
Organism Saccharomyces cerevisiae
Characteristics Rap1 IP on Nrg1del #2 during log phase
Treatment protocol Rich Media (YPD 2% Glucose)
Growth protocol Expontential Growth (OD 0.6-1.0)
Extracted molecule genomic DNA
Extraction protocol IP DNA isoloated from 1mg protein from WCE by IP with AB Y-300.
Label Cy5
Label protocol samples and references were amplified using a random primed PCR-based method (see Bohlander et al., 1992)
 
 
Hybridization protocol Direct hybridization 4X SSC, 0.2% SDS
Scan protocol Images were acquired using a GenePix 4000B scanner and Genepix software (Axon Instruments)
Description Rap1 ChIP-chip using RAP1-AB (y-300) on nrg1del culture # 2 during expontential growth OD 0.8993.Strain - nrg1del, BY4741
Data processing The normalized median log2 ratios (ch2/ch1) were download from UNC microarray database, dye swapped experiments were inverted
 
Submission date Oct 24, 2006
Last update date Jan 23, 2007
Contact name Jason D. Lieb
E-mail(s) jlieb@bio.unc.edu
Phone 919-843-3228
Organization name Univeristy of North Carolina at Chapel Hill
Department Biology
Lab Lieb
Street address CB 3280
City Chapel Hill
State/province NC
ZIP/Postal code 27599
Country USA
 
Platform ID GPL4414
Series (1)
GSE5948 A chromatin-mediated mechanism for specification of conditional transcription factor targets

Data table header descriptions
ID_REF UNC Unique ID from UNC microarray database
VALUE normalized log2 (Median of Ratios) IP/Input

Data table
ID_REF VALUE
302 -2.029
303 -0.211
304 -0.37
305 0.257
306 1.236
307 -0.582
308 -0.974
309 -0.152
310 -1.18
311 1.179
312 -0.388
313 -3.252
314 -0.839
315 -0.189
316 0.089
317 -0.826
318 -0.281
319 0.869
320 -1.761
321 -0.313

Total number of rows: 15397

Table truncated, full table size 174 Kbytes.




Supplementary file Size Download File type/resource
GSM142104.gpr.gz 1.7 Mb (ftp)(http) GPR

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