|
| Status |
Public on Jun 28, 2014 |
| Title |
Sus_scrofa_CB_expr_HX12_CTL3 |
| Sample type |
RNA |
| |
|
| Source name |
intestinal epithelial cell line
|
| Organism |
Sus scrofa |
| Characteristics |
cell line: IPEC-J2
|
| Treatment protocol |
No treatment
Cells grown to confluence , and then 12.5g/ml PR-39 were added into wells and cultured with cells for 4 hours.
|
| Growth protocol |
IPEC-J2 cells were grown in Hams F-12 medium supplemented with 5% fetal bovine serum and maintained in an atmosphere of 5% CO2 at 37
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy Mini kit (Qiagen Inc., Valencia, CA, USA) and the RNAprotect reagent (Qiagen) and DNA was removed by on-column DNase digestion with the RNase-Free DNase set (Qiagen). RNA quality and concentration was determined by analysis with an Agilent 2100 bioanalyzer at the Harvard-Partners Center for Genetics and Genomics (Harvard Medical School, Cambridge, MA USA).
|
| Label |
Cy3
|
| Label protocol |
Labeling was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
|
| |
|
| Hybridization protocol |
Hybridization was performed by NimbleGen Systems Inc., Madison, WI, USA following their standard operating protocol. See www.nimblegen.com.
|
| Scan protocol |
Scanning was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
|
| Description |
The IPEC-J2 cell line is a non-transformed intestinal cell line originally derived from jejunal epithelia isolated from a neonatal, unsuckled piglet
|
| Data processing |
The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249), quantile normalization (Bolstad et al. Bioinformatics 19(2):185), and background correction as implemented in the NimbleScan software package, version 2.4.27 (Roche NimbleGen, Inc.).
|
| |
|
| Submission date |
Jun 27, 2014 |
| Last update date |
Jun 28, 2014 |
| Contact name |
Xi Xia |
| E-mail(s) |
ivy880425@gmail.com
|
| Organization name |
Zhejiang University
|
| Department |
Institute of Feed Science
|
| Street address |
866 Yuhangtang Road
|
| City |
Hangzhou |
| State/province |
Zhejiang |
| ZIP/Postal code |
310058 |
| Country |
China |
| |
|
| Platform ID |
GPL18875 |
| Series (1) |
| GSE58876 |
Effect of antimicrobial peptide PR-39 on the gene expression of porcine intestinal epithelial cell line (IPEC-J2) |
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