|
| Status |
Public on Jul 29, 2014 |
| Title |
oxidative stress (20min) - WT / WT+0.4mM H2O2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
mid-log
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: ACY044
treatment: mid-log
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
as described in Capaldi et al (2008). Nature Genetics 40, 1300 - 1306 (2008). doi:10.1038/ng.235
|
| Label |
Cy5
|
| Label protocol |
as described in Capaldi et al (2008). Nature Genetics 40, 1300 - 1306 (2008). doi:10.1038/ng.235
|
| |
|
| Channel 2 |
| Source name |
Oxidative stress, 0.4mM H2O2, 20min
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: ACY044
treatment: Oxidative stress, 0.4mM H2O2, 20min
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
as described in Capaldi et al (2008). Nature Genetics 40, 1300 - 1306 (2008). doi:10.1038/ng.235
|
| Label |
Cy3
|
| Label protocol |
as described in Capaldi et al (2008). Nature Genetics 40, 1300 - 1306 (2008). doi:10.1038/ng.235
|
| |
|
| |
| Hybridization protocol |
as described in Capaldi et al (2008). Nature Genetics 40, 1300 - 1306 (2008). doi:10.1038/ng.235
|
| Scan protocol |
Microarrays scanned on Genepix 4000b. Images were quantified with Genepix Pro software version 6.1.
|
| Description |
251632210313-1 is a dye swap
|
| Data processing |
Background subtraction and filtering (regression correlation >0.6 AND mean intensity/median background intensity is >1.5-fold in EITHER channel) performed by Stanford Microarray Database version 2.14 (Nucl. Acids Res. (2007) 35 (suppl 1): D766-D770. doi: 10.1093/nar/gkl1019).
|
| |
|
| Submission date |
Jul 01, 2014 |
| Last update date |
Jul 30, 2014 |
| Contact name |
James E Hughes Hallett |
| Organization name |
University of Arizona
|
| Department |
Molecular and Cellular Biology
|
| Lab |
Capaldi
|
| Street address |
1007 E. Lowell Street
|
| City |
Tucson |
| State/province |
AZ |
| ZIP/Postal code |
85721 |
| Country |
USA |
| |
|
| Platform ID |
GPL16244 |
| Series (1) |
| GSE58992 |
State Transitions in the TORC1 Signaling Pathway and Information Processing in S. cerevisiae |
|
