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Status |
Public on Dec 31, 2018 |
Title |
C42B_control_2 |
Sample type |
SRA |
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Source name |
prostate cancer cell line
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Organism |
Homo sapiens |
Characteristics |
tissue: prostate cell line: prostate cancer cell line C42B treatment: control
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Treatment protocol |
C42B cells were treated with 1uM SP-2509 or 0.1% DMSO the day after plating, and harvested 72hrs post-treatment. PC3 cells were treated with 2uM SP-2509 or 0.1% DMSO the day after plating, and harvested 72hrs post-treatment. LNCaP cells were treated with 1uM SP-2509 or 0.1% DMSO the day after plating, and harvested 72hrs post-treatment.
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Growth protocol |
C42B cells were plated in triplicate in 6-well plates, 250,000 cells/well in RPMI+10% charcoal-stripped FBS. PC3 cells were plated in triplicate in 6-well plates, 250,000 cells/well in RPMI+10% FBS. LNCaP cells were plated in triplicate in 6-well plates, 350,000 cells/well in RPMI+10% FBS.
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Extracted molecule |
total RNA |
Extraction protocol |
TriZol+CHCl3 Agilent SureSelect Stranded RNAseq
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Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2000 |
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Description |
Sample 2
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Data processing |
Tophat version 2.0.9 for alignment Cufflinks version 2.1.1 for transcript assembly Genome_build: hg19 Supplementary_files_format_and_content: Text file with fpkm-like measurement for each UCSC (v. hg19) transcript
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Submission date |
Jul 02, 2014 |
Last update date |
May 15, 2019 |
Contact name |
Joshi Alumkal |
Organization name |
Knight Cancer Institute
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Street address |
3303 SW Bond Ave. CH14R
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City |
Portland |
State/province |
OR |
ZIP/Postal code |
97239 |
Country |
USA |
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Platform ID |
GPL11154 |
Series (1) |
GSE59009 |
RNA-seq profiles after SP-2509 Treatment of LNCaP, C42B, and PC3 cells |
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Relations |
BioSample |
SAMN02899693 |
SRA |
SRX643498 |
Supplementary file |
Size |
Download |
File type/resource |
GSM1424421_Sample_RNA130912JA_3.22.13_C42B_2509_0_2.txt.gz |
453.9 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
Raw data are available in SRA |
Processed data provided as supplementary file |
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