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Sample GSM1429327 Query DataSets for GSM1429327
Status Public on Oct 07, 2015
Title HCC33_Scramble5
Sample type RNA
 
Source name Small Cell Lung Cancer (SCLC)
Organism Homo sapiens
Characteristics cell line: HCC33
shRNA: transfected with Scramble 5 (short hairpin RNA number 5 doubly mutated)
Treatment protocol Not special additional treatment
Growth protocol Cells where growth on RPMI media with 10% FBS, 1% PS
Extracted molecule total RNA
Extraction protocol Total mRNA was extrated using Trizol Reagent.
Label Cy3
Label protocol Amount of nucleic acid labeled: 100ng. Commercial One-Color Microarray-Based Gene Expression Analysis (Low Input Quick Amp Labeling) version 6.5 kit by following manufacturer instructions. Agilent manual G4140-90040 of May 2010. Amplification: by RNA polymerases. Briefly, MMLV-RT retrotranscription of sample from a T7 promoter primer is followed by a T7 RNA pol catalysed in vitro transcription reaction in the presence of Cy3-CTP fluorophore. Labeled samples are purified with silica-based spin columns.
 
Hybridization protocol Microarray: Human Gene Expression G3 v2 60K (Agilent microarray design ID 039494, P/N G4851B). Microarray sequences and annotations are listed in sheet '039494_hg19_20120628'. Hybridization chamber type: SureHyb hybridization chamber (Agilent). Quantity of labeled extract used: 600 ng. Volume: 50 µL. Temperature (ºC): 65. Duration: 17 hours.
Scan protocol Scanned on an G2505C DNA microarray scanner (Agilent).
Description HCC33 cell line transfected with Scramble 5 (short hairpin RNA number 5 doubly mutated)
Data processing Images were analysed by Agilent Feature Extraction Software (ver. 11.5), which performed feature quantitation and additive detrend correction. No background subtraction was performed.
 
Submission date Jul 07, 2014
Last update date Apr 23, 2018
Contact name Manel Esteller
Organization name IDIBELL
Department PEBC
Lab Cancer Epigenetics
Street address Hospital Duran i Reynals Av. Gran Via s/n km, 2.7
City L'Hospitalet de Llobregat
State/province Barcelona
ZIP/Postal code 08908
Country Spain
 
Platform ID GPL17077
Series (2)
GSE59136 Identification of KAT6B signature on KAT6B deficient SCLC cell Lines
GSE62775 KAT6B gene deletion as a predictive biomarker to Irinotecan response in small cell lung cancer
Relations
Reanalyzed by GSE113533

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
GE_BrightCorner 15.8853923
DarkCorner 4.757999365
A_23_P117082 11.40032243
A_33_P3246448 4.904013124
A_33_P3318220 4.804271697
A_33_P3236322 5.152395357
A_33_P3319925 5.301595851
A_21_P0000509 16.34107578
A_21_P0000744 10.31168046
A_24_P215804 6.666262701
A_23_P110167 9.629104236
A_33_P3211513 8.208552493
A_23_P103349 4.980010397
A_32_P61480 5.231325811
A_33_P3788124 4.948494524
A_33_P3414202 8.781573558
A_33_P3316686 7.48333392
A_33_P3300975 5.31071181
A_33_P3263061 11.60923772
A_33_P3261373 5.036293389

Total number of rows: 50739

Table truncated, full table size 1260 Kbytes.




Supplementary file Size Download File type/resource
GSM1429327_253949430077_S01_GE1_1105_Oct12_B_2_3.txt.gz 3.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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