|
| Status |
Public on Dec 01, 2019 |
| Title |
Control_2 |
| Sample type |
RNA |
| |
|
| Source name |
Hepatoma cell
|
| Organism |
Rattus norvegicus |
| Characteristics |
cell line: FaO
cell type: hepatocyte
|
| Treatment protocol |
Fao cells were treated in the presence of 2% FCS and treated with a single dose of 0.1µM Thapsigargin and daily with 500 µM α-Lipoic acid. RNA was extracted after treatment with TG for 6 and 18 hrs and after treatment with α-Lipoic acid for 6, 18, 24, 30, 42, 48 and72 hrs. Three wells for each sample were pooled
|
| Growth protocol |
Hepatoma cells FaO were grown in DMEM Plus Glutamax I (Invitrogen, Life Technologies) supplemented with Pen Strep and 10% heat-inactivated FCS (Life Technologies) in a humidified atmosphere of 5% CO2/95% air, at 37°C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Hepatoma cell FaO
|
| Data processing |
The data were normalised using quantile normalisation with Illumina BeadStudio 3.0.19.0 software (Illumina Inc) and BRB Array Tools (Version 4.2.0)
|
| |
|
| Submission date |
Jul 07, 2014 |
| Last update date |
Dec 01, 2019 |
| Contact name |
Gabriella Simbula |
| E-mail(s) |
gsimbula@unica.it
|
| Organization name |
THE UNIVERSITY OF CAGLIARI
|
| Street address |
Porcel 4
|
| City |
Cagliari |
| State/province |
CA |
| ZIP/Postal code |
09124 |
| Country |
Italy |
| |
|
| Platform ID |
GPL6101 |
| Series (1) |
| GSE59164 |
Lipoic acid induced endoplasmic reticulum stress-mediated apoptosis in rat hepatoma cells:identification of new ER stress aggravator (ERSA) |
|
