|
| Status |
Public on Oct 30, 2006 |
| Title |
3T3 / Reference |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
3T3
|
| Organism |
Mus musculus |
| Characteristics |
3T3
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
|
| Label |
Cy3
|
| Label protocol |
Direct incorporation of CyDye labeled dCTP by reverse transcription.
|
| |
|
| Channel 2 |
| Source name |
Mouse Embryo Reference
|
| Organism |
Mus musculus |
| Characteristics |
Reference
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
|
| Label |
Cy5
|
| Label protocol |
Direct incorporation of CyDye labeled dCTP by reverse transcription.
|
| |
|
| |
| Hybridization protocol |
Hybridization was performed at 50C and 35% formamide for 16 hours.
|
| Scan protocol |
Microarrays were scanned using the Agilent G2505B scanner.
|
| Description |
Gene expression data from cell type: 3T3
|
| Data processing |
log2 ratio of Cy5/Cy3
|
| |
|
| Submission date |
Oct 30, 2006 |
| Last update date |
Feb 13, 2007 |
| Contact name |
Sheri Holmen |
| E-mail(s) |
sholmen@nvcancer.org
|
| Phone |
702-822-5295
|
| Fax |
702-944-0473
|
| URL |
http://www.nevadacancerinstitute.org/
|
| Organization name |
Nevada Cancer Institute
|
| Department |
Drug Development
|
| Lab |
Holmen Lab
|
| Street address |
10441 W. Twain Ave.
|
| City |
Las Vegas |
| State/province |
NV |
| ZIP/Postal code |
89135 |
| Country |
USA |
| |
|
| Platform ID |
GPL4487 |
| Series (1) |
| GSE6194 |
Oncogenic potential of RAS isoforms in melanocytes |
|
