|
Status |
Public on Feb 24, 2015 |
Title |
Panc1 rep-2 |
Sample type |
RNA |
|
|
Source name |
Panc1 cell line
|
Organism |
Homo sapiens |
Characteristics |
cell line: Panc1 cell type: dasatinib-resistant pancreatic cancer cell line
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
Label |
Biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
replicate 2 5957936057_H
|
Data processing |
The data were normalised using quantile normalisation and log2 transformed with 'beadarray' Bioconductor package in R
|
|
|
Submission date |
Jul 11, 2014 |
Last update date |
Feb 24, 2015 |
Contact name |
Phillip H Koeffler |
E-mail(s) |
phillip_koeffler@nuhs.edu.sg
|
Organization name |
Cancer Science Institute
|
Lab |
H. Phillip Koeffler's lab
|
Street address |
14 Medical Drive
|
City |
Singapore |
ZIP/Postal code |
117599 |
Country |
Singapore |
|
|
Platform ID |
GPL10558 |
Series (1) |
GSE59357 |
Gene expression profiles of dasatinib-resistant and dasatinib-sensitive pancreatic cancer cell lines |
|