|
| Status |
Public on Jul 31, 2015 |
| Title |
Hemocytes after 31 days challenge with trophozoites suspension_3 |
| Sample type |
RNA |
| |
|
| Source name |
31 days after a Perkinsus olseni challenge
|
| Organism |
Ruditapes philippinarum |
| Characteristics |
cell type: hemocytes
pathogen: Perkinsus olseni
|
| Treatment protocol |
Rphilippinarum injected (intramuscular) with 100 µl of filtered sea water (FSW) or trophozoites suspension. Hemocytes were collected 5, 10, 14, or 31 days after challenge.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extracted according to QiAgen RNeasy MiniKit protocol for tissue samples
|
| Label |
Cyanine 3
|
| Label protocol |
Amplified and labeled according to Low Input Quick Amp labeling kit (Agilent Technologies)
|
| |
|
| Hybridization protocol |
Hybridized according to Agilent One-Color Microarray-Based Gene Expression Analysis protocol
|
| Scan protocol |
Array was scanned according to standard Agilent protocol at a resolution of 5 microns modifying default setting to scan the slide twice at two different sensitivity settings (XDRHi 100% and XDRLo 10%)
|
| Description |
hemocytes RNA of Ruditapes philippinarum
Healthy and Perkinsus-infected clams (V. philippinarum) were obtained from Carril and Pontevedra shellfish farms, respectively (Galicia, NW Spain).Healthy clams were maintained in an open circuit filtered sea water tanks at 15°C with aeration.All animals were fed daily with a mixture of microalgae containing Phaeodactylum tricornutum, Isochrysis galbana and Rhodomonas lens. Clams were acclimatized to the aquarium conditions for one week before the experiments were conducted.
|
| Data processing |
Data processed with FeatureExtraction v9.5 and normalized using Gene-Spring GX version 11.0.2 software (Agilent Technologies)
|
| |
|
| Submission date |
Jul 14, 2014 |
| Last update date |
Aug 01, 2015 |
| Contact name |
Massimo Milan |
| E-mail(s) |
massimo.milan@unipd.it
|
| Phone |
+39 0498272941
|
| Organization name |
University of Padova
|
| Department |
Dept. of Department of Public Health, Comparative Pathology,and Veterinary Hygiene
|
| Street address |
Viale dell'Università 16
|
| City |
Legnaro |
| State/province |
PD |
| ZIP/Postal code |
35020 |
| Country |
Italy |
| |
|
| Platform ID |
GPL16450 |
| Series (1) |
| GSE59399 |
Gene expression profile of hemocytes from Manila clam (Venerupis philippinarum) after a Perkinsus olseni challenge using an immune-enriched oligo-microarray |
|
