|
| Status |
Public on Feb 01, 2015 |
| Title |
P493-6_c-myc off_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
P496-6 cells, Tetracycline+, t=72h
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: P493-6 B
|
| Treatment protocol |
For repression of Myc in P493-6, 0,1µg/mL of Tetracycline Hydrochloride (UMCG Pharmacy, Groningen, NL) was added to the culture medium for 72h. For Myc re-induction the cells were spun down (200rcf, 5min) and re-suspended in fresh, tetracycline-free, complete medium for the indicated time.
|
| Growth protocol |
RPMI-1640 medium supplemented with 100U/ml penicillin, 0.1mg/ml streptomycin, 2mM ultraglutamine and 10% Fetal Calf Serum (all Cambrex Biosciences, Walkersville, MD, USA), 37⁰C, 5% CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Standard Trizol RNA isolation (Life Technologies)
|
| Label |
Cy3
|
| Label protocol |
Cy3 labelled using 50-100ng total RNA in combination with the LowInput QuickAmp Labelling Kit, Cyanine 3 CTP Dye Pack and RNA Spike-In kit (all Agilent Technologies).
|
| |
|
| Hybridization protocol |
According to Manufacturers instructions
|
| Scan protocol |
Agilent DNA Microarray Scanner, Agilent Feature Extraction software version 10.7.3.1.
|
| Description |
mRNA and lncRNA expression analysis_P493-6 B cells_Tetracycline treated 72h
|
| Data processing |
GeneSpring GX 12.5 software (Agilent Technologies); quantile normalization without base line transformation.
|
| |
|
| Submission date |
Jul 16, 2014 |
| Last update date |
Feb 01, 2015 |
| Contact name |
Melanie Winkle |
| E-mail(s) |
melanie.winkle@gmail.com
|
| Organization name |
UMC Groningen
|
| Department |
Pathology & Medical Biology
|
| Lab |
van den Berg
|
| Street address |
Hanzeplein 1
|
| City |
Groningen |
| State/province |
Groningen |
| ZIP/Postal code |
9713 GZ |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL18959 |
| Series (2) |
| GSE59477 |
Gene expression analysis to identify Myc-regulated long noncoding RNAs in B cells with variable Myc levels |
| GSE59480 |
Long noncoding RNAs as a novel component of the Myc transcriptional network |
|
