|
| Status |
Public on Nov 15, 2014 |
| Title |
Induced beta cell d60_2 |
| Sample type |
RNA |
| |
|
| Source name |
Pancreas_induced beta cell_d60
|
| Organism |
Mus musculus |
| Characteristics |
strain: Rag1-/-
tissue: Pancreas
cell type: Induced beta cell d60
|
| Treatment protocol |
Pancreata were perfused through the common bile duct. After removing endogenous islets, the acinar fraction was digested with liberase and Elastase (Roche) into single cells.
|
| Growth protocol |
Rag1-/- were obtained from Jackson Labs. For β- cell induction, 100ul (>1x 109 pfu) of purified adenovirus was directly injected into the splenic lobe of the dorsal pancreas.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cherry+ induced beta cells were isolated by fluorescent activated cell sorting (FACS) with FACSaria (BD Bioscience), RNA samples were extracted by Trizol
|
| Label |
biotin
|
| Label protocol |
label protocol for Illumina MouseRef-8 v2.0 expression beadchip
|
| |
|
| Hybridization protocol |
Hyb protocol for Illumina MouseRef-8 v2.0 expression beadchip
|
| Scan protocol |
Scan protocol for Illumina MouseRef-8 v2.0 expression beadchip
|
| Description |
replicate 2
|
| Data processing |
The raw signal was transformed to logarithm scale (log10).
|
| |
|
| Submission date |
Jul 16, 2014 |
| Last update date |
Nov 15, 2014 |
| Contact name |
Kendell Clement |
| Organization name |
Harvard University
|
| Lab |
Meissner Lab
|
| Street address |
7 Divinity Ave.
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02138 |
| Country |
USA |
| |
|
| Platform ID |
GPL18965 |
| Series (1) |
| GSE59490 |
Transcriptional and epigenetic program changes of induced beta cells over time [expression array] |
|
