|
| Status |
Public on Aug 01, 2014 |
| Title |
315R |
| Sample type |
RNA |
| |
|
| Source name |
p65-/-Ras
|
| Organism |
Mus musculus |
| Characteristics |
cell type: MEF
genotype: p65-/-Ras
|
| Treatment protocol |
p65+/+ and p65-/- MEFs were infected with pBabe-HRASG12V-Hygro to make p65+/+Ras and p65-/-Ras cells. Then p65+/+Ras or p65-/-Ras cells were injected into SCID mice. After tumor appearance, tumors were removed and put back into culture after collagenase treatment and selected with Hygromycin for 2 weeks. Then total RNA were isolated from cells before or after tumor formation
|
| Growth protocol |
DMEM + 10%FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol total RNA isolation
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Data processing |
dCHIP. Log2 ratios are available on the series level.
|
| |
|
| Submission date |
Jul 17, 2014 |
| Last update date |
Aug 01, 2014 |
| Contact name |
David Jingxin Wang |
| E-mail(s) |
jingxin.wang@osumc.edu
|
| Phone |
6146884507
|
| Organization name |
The Ohio State University
|
| Department |
Comprehensive Cancer Center
|
| Lab |
Guttridge Lab
|
| Street address |
BRT536, 460w, 12th Ave.
|
| City |
Columbus |
| State/province |
Ohio |
| ZIP/Postal code |
43210 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
|
