|
| Status |
Public on Jul 23, 2014 |
| Title |
MiR-21 knockout replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
MiR-21-KO
|
| Organism |
Mus musculus |
| Characteristics |
tissue: colon mucosa
strain: c57BL/6
genotype: MiR-21 knockout
group: MiR-21-KO
|
| Treatment protocol |
MiR-21-/- (21KO) mice on C57BL/6 background were kindly provided by Dr. Eric Olson (Patrick, et al. J Clin Invest. 2010;120:3912-3916.). The miR-21-/- mice do not develop spontaneous colitis. Gene expression profiles were established for normal miR-21-/- mice and wild type c57BL/6 mice (WT) from Taconic Farm Inc. (Hudson, NY)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
not provided
|
| |
|
| Hybridization protocol |
Hybridized to Illumina MouseRef-8 v2.0 expression beadchip.
|
| Scan protocol |
not provided
|
| Description |
JK11
|
| Data processing |
raw data were processed using the R/Bioconductor package limma for background correction and quantile normalization
|
| |
|
| Submission date |
Jul 22, 2014 |
| Last update date |
Jul 23, 2014 |
| Contact name |
Yong Huang |
| E-mail(s) |
yh9fj@virginia.edu
|
| Phone |
(434) 243-0842
|
| Organization name |
University of Viginia
|
| Department |
Medicine
|
| Street address |
1340 Jefferson Park Ave
|
| City |
Charlottesville |
| State/province |
VA |
| ZIP/Postal code |
22908 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (2) |
| GSE59648 |
Divergent influence of microRNA-21 deletion on murine colitis phenotypes [mRNA] |
| GSE59651 |
Divergent influence of microRNA-21 deletion on murine colitis phenotypes |
|
