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Sample GSM144733 Query DataSets for GSM144733
Status Public on Nov 16, 2006
Title Ronghe 062205 1
Sample type RNA
 
Source name Rat skin keratinocyte without treatment
Organism Rattus norvegicus
Characteristics Dorsal skin of neonatal male weanling Sprague_Dawley rats(CD)
Treatment protocol No treatment
Growth protocol N/A
Extracted molecule polyA RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.5-2ug polyA RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
Description An immortalized rat keratinocyte cell line,originally isolated from the dorsal skin of neonatal male weanling Sprague_Dawley rats(CD)
Data processing The data were analyzed with BRB-ArraYTools version 3.5.0-Beta-1
 
Submission date Nov 16, 2006
Last update date Nov 16, 2006
Contact name Ronghe Zhang
E-mail(s) zhangr@env.med.nyu.edu
Phone 8457313584
Fax 8453515472
Organization name New York University School of Medicine
Department Environmental Medicine
Lab Fred Burns
Street address 57 Old Forge Rd
City Tuxedo Park
State/province NY
ZIP/Postal code 10987
Country USA
 
Platform ID GPL1355
Series (1)
GSE6299 Gene expression and cell cycle arrest in a rat keratinocyte cell line exposed to 56 Fe Ions

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 54.5 P 0.000972
AFFX-BioB-M_at 71.2 P 0.000662
AFFX-BioB-3_at 36.4 P 0.000509
AFFX-BioC-5_at 175.4 P 0.000081
AFFX-BioC-3_at 184.8 P 0.000052
AFFX-BioDn-5_at 308.2 P 0.000754
AFFX-BioDn-3_at 633.9 P 0.000225
AFFX-CreX-5_at 1375.3 P 0.000044
AFFX-CreX-3_at 1750.9 P 0.000044
AFFX-DapX-5_at 1.9 A 0.455409
AFFX-DapX-M_at 1.1 A 0.712257
AFFX-DapX-3_at 0.5 A 0.910541
AFFX-LysX-5_at 2.3 A 0.645559
AFFX-LysX-M_at 2.9 A 0.603089
AFFX-LysX-3_at 5.5 M 0.050229
AFFX-PheX-5_at 0.4 A 0.84329
AFFX-PheX-M_at 2.3 A 0.574038
AFFX-PheX-3_at 7.7 A 0.514891
AFFX-ThrX-5_at 1.6 A 0.617401
AFFX-ThrX-M_at 0.5 A 0.932339

Total number of rows: 31099

Table truncated, full table size 814 Kbytes.




Supplementary file Size Download File type/resource
GSM144733.CEL.gz 2.4 Mb (ftp)(http) CEL

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