|
Status |
Public on Nov 16, 2006 |
Title |
Ronghe 062205 11 |
Sample type |
RNA |
|
|
Source name |
Rat skin keratynocyte with radiation treatment
|
Organism |
Rattus norvegicus |
Characteristics |
Dorsal skin of neonatal male weanling Sprague_Dawley rats(CD)
|
Treatment protocol |
3Gy 56 Fe radiation treatment
|
Growth protocol |
N/A
|
Extracted molecule |
polyA RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.5-2ug polyA RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
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|
|
Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
|
Description |
An immortalized rat keratinocyte cell line,originally isolated from the dorsal skin of neonatal male weanling Sprague_Dawley rats(CD)
|
Data processing |
The data were analyzed with BRB-ArraYTools version 3.5.0-Beta-1
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|
|
Submission date |
Nov 16, 2006 |
Last update date |
Nov 16, 2006 |
Contact name |
Ronghe Zhang |
E-mail(s) |
zhangr@env.med.nyu.edu
|
Phone |
8457313584
|
Fax |
8453515472
|
Organization name |
New York University School of Medicine
|
Department |
Environmental Medicine
|
Lab |
Fred Burns
|
Street address |
57 Old Forge Rd
|
City |
Tuxedo Park |
State/province |
NY |
ZIP/Postal code |
10987 |
Country |
USA |
|
|
Platform ID |
GPL1355 |
Series (1) |
GSE6299 |
Gene expression and cell cycle arrest in a rat keratinocyte cell line exposed to 56 Fe Ions |
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