|
| Status |
Public on Nov 16, 2006 |
| Title |
Ronghe 062205 12 |
| Sample type |
RNA |
| |
|
| Source name |
Rat skin keratynocyte with radiation treatment
|
| Organism |
Rattus norvegicus |
| Characteristics |
Dorsal skin of neonatal male weanling Sprague_Dawley rats(CD)
|
| Treatment protocol |
3Gy 56 Fe radiation treatment
|
| Growth protocol |
N/A
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.5-2ug polyA RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
|
| Description |
An immortalized rat keratinocyte cell line,originally isolated from the dorsal skin of neonatal male weanling Sprague_Dawley rats(CD)
|
| Data processing |
The data were analyzed with BRB-ArraYTools version 3.5.0-Beta-1
|
| |
|
| Submission date |
Nov 16, 2006 |
| Last update date |
Nov 16, 2006 |
| Contact name |
Ronghe Zhang |
| E-mail(s) |
zhangr@env.med.nyu.edu
|
| Phone |
8457313584
|
| Fax |
8453515472
|
| Organization name |
New York University School of Medicine
|
| Department |
Environmental Medicine
|
| Lab |
Fred Burns
|
| Street address |
57 Old Forge Rd
|
| City |
Tuxedo Park |
| State/province |
NY |
| ZIP/Postal code |
10987 |
| Country |
USA |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE6299 |
Gene expression and cell cycle arrest in a rat keratinocyte cell line exposed to 56 Fe Ions |
|
